ArticleEcology and evolution2018
SSR-seq: Genotyping of microsatellites using next-generation sequencing reveals higher level of polymorphism as compared to traditional fragment size scoring.
Article in Ecology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Upscaling Genotyping by Amplicon Sequencing With GBAS-GUI.Molecular ecology resources · 2026Article
- Bridging the gap between legacy polymerase chain reaction-based microsatellite data with high-throughput sequencing data for conservation genomics.The Journal of heredity · 2026Article
- Genetic differentiation of the supralittoral gastropod Tectarius striatus (North Atlantic Archipelagos) and development of new microsatellite resources.BMC ecology and evolution · 2026Article
- Article
- Structural Characteristics, Comparative Analyses, and Conservation Significance of the Complete Chloroplast Genome of the Critically EndangeredEcology and evolution · 2026Article
- Article
- Flow Cytometric Seed Screening and Seed Genotyping: Methods for Detecting Reproductive Modes in Flowering Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Estimating asian elephant abundance: a comparative analysis of dung counts and genetic SECR in a known population of Kodagu, Karnataka, India.BMC ecology and evolution · 2025Article
- First Assembly of a Draft Genome of the Critically Endangered Northern Muriqui (Ecology and evolution · 2025Article
- Population Dynamics ofEcology and evolution · 2025Article
- Genetic Conservation and Population Management of Non-Human Primates: Parentage Determination Using Seven Microsatellite-Based Multiplexes.Ecology and evolution · 2025Article
- A novel strategy to study apomixis, automixis, and autogamy in plants.Plant reproduction · 2024Article
- Article
- Data of SSRs primers for high-throughput genotyping-by-sequencing (SSR-Seq) based on the partial genome assembly ofData in brief · 2023Article
- Genome survey and genetic characterization ofFrontiers in plant science · 2023Article
- Phenotypic and genetic characterization of anFrontiers in plant science · 2023Article
- Article
- The Maternal Donor ofFrontiers in plant science · 2022Article
- Development of SSR Databases Available for Both NGS and Capillary Electrophoresis in Apple, Pear and Tea.Plants (Basel, Switzerland) · 2021Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microsatellites (or simple sequence repeats, SSR) are widely used markers in population genetics. Traditionally, genotyping was and still is carried out through recording fragment length. Now, next-generation sequencing (NGS) makes it easy to obtain also sequence information for the loci of interest. This avoids misinterpretations that otherwise could arise due to size homoplasy. Here, an NGS strategy is described that allows to genotype hundreds of individuals at many custom-designed SSR loci simultaneously, combining multiplex PCR, barcoding, and Illumina sequencing. We created three different datasets for which alleles were coded according to (a) length of the repetitive region, (b) total fragment length, and (c) sequence identity, in order to evaluate the eventual benefits from having sequence data at hand, not only fragment length data. For each dataset, genetic diversity statistics, as well as
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.