ArticleStem cells and cloning : advances and applications2018
A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.
Article in Stem cells and cloning : advances and applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 8 citations in OpenAlex.
- A scalable human iPSC-based neuromuscular disease model on suspended biobased elastomer nanofiber scaffolds.Biofabrication · 2023Article
- 3D Compartmentalised Human Pluripotent Stem Cell-derived Neuromuscular Co-cultures.Bio-protocol · 2023Article
- Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing.Frontiers in integrative neuroscience · 2022Article
- Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle.PloS one · 2022Article
- Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors.Physiological reports · 2021Article
- A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction.Journal of clinical medicine · 2020Article
- MicroRNA and mRNA profiling in the idiopathic inflammatory myopathies.BMC rheumatology · 2020Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough considerable research on neuromuscular junctions (NMJs) has been conducted, the prospect of in vivo NMJ studies is limited and these studies are challenging to implement. Therefore, there is a clear unmet need to develop a feasible, robust, and physiologically relevant in vitro NMJ model.
objectiveWe aimed to establish a novel functional human NMJs platform, which is serum and neural complex media/neural growth factor-free, using human immortalized myoblasts and human embryonic stem cells (hESCs)-derived neural progenitor cells (NPCs) that can be used to understand the mechanisms of NMJ development and degeneration.
methodsImmortalized human myoblasts were co-cultured with hESCs derived committed NPCs. Over the course of the 7 days myoblasts differentiated into myotubes and NPCs differentiated into motor neurons.
resultsNeuronal axon sprouting branched to form multiple NMJ innervation sites along the myotubes and the myotubes showed extensive, spontaneous contractile activity. Choline acetyltransferase and βIII-tubulin immunostaining confirmed that the NPCs had matured into cholinergic motor neurons. Postsynaptic site of NMJs was further characterized by staining dihydropyridine receptors, ryanodine receptors, and acetylcholine receptors by α-bungarotoxin.
conclusionWe established a functional human motor unit platform for in vitro investigations. Thus, this co-culture system can be used as a novel platform for 1) drug discovery in the treatment of neuromuscular disorders, 2) deciphering vital features of NMJ formation, regulation, maintenance, and repair, and 3) exploring neuromuscular diseases, age-associated degeneration of the NMJ, muscle aging, and diabetic neuropathy and myopathy.
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