ArticleScientific reports2018
Analysis of genome-wide variants through bulked segregant RNA sequencing reveals a major gene for resistance to Plasmodiophora brassicae in Brassica oleracea.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 68 citations in OpenAlex.
- Synthetic Raphanobrassica Genome Reveals Functional and Evolutionary Insights Into Clubroot Resistance Genes on Chromosome R5.Plant biotechnology journal · 2026Article
- Genetic and Epigenetic Mechanisms Underpinning Biotic Stress Resilience ofPlants (Basel, Switzerland) · 2025Review
- Identification and characterization ofHorticulture research · 2025Article
- Genome-Wide Identification and Functional Evolution of NLR Gene Family inCurrent issues in molecular biology · 2025Article
- Identification of Rcr12, a single dominant clubroot resistance gene near Rcr6 on chromosome B3 of Brassica nigra.BMC plant biology · 2025Article
- Clubroot resistant in cruciferous crops: recent advances in genes and QTLs identification and utilization.Horticulture research · 2025Article
- Identification of three novel QTL for resistance to highly aggressive Canadian strains ofFrontiers in plant science · 2025Article
- Genetic improvement of clubroot resistance in the recessive genic male sterile line YA001 inFrontiers in plant science · 2025Article
- Comprehensive review ofFrontiers in microbiology · 2025Review
- Optimizing Clubroot Management and the Role of Canola Cultivar Mixtures.Pathogens (Basel, Switzerland) · 2024Review
- Resynthesizing Brassica napus with race specific resistance genes and race non-specific QTLs to multiple races of Plasmodiophora brassicae.Scientific reports · 2024Article
- RNA-Seq Bulked Segregant Analysis of an ExoticInternational journal of molecular sciences · 2024Article
- Map-based cloning and functional analysis of a major quantitative trait locus, BolC.Pb9.1, controlling clubroot resistance in a wild Brassica relative (Brassica macrocarpa).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Identification of QTLs for resistance to 10 pathotypes of Plasmodiophora brassicae in Brassica oleracea cultivar ECD11 through genotyping-by-sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Review
- Identification of a genomic region containing genes involved in resistance to four pathotypes of Plasmodiophora brassicae in Brassica rapa turnip ECD02.The plant genome · 2022Article
- Multi-Omics Approaches to Improve Clubroot Resistance in Brassica with a Special Focus onInternational journal of molecular sciences · 2022Review
- Article
- Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding.Frontiers in genetics · 2022Review
- Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping.Scientific reports · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two cabbage (Brassica oleracea) cultivars 'Tekila' and 'Kilaherb' were identified as resistant to several pathotypes of Plasmodiophora brassicae. In this study, we identified a clubroot resistance gene (Rcr7) in 'Tekila' for resistance to pathotype 3 of P. brassicae from a segregating population derived from 'Tekila' crossed with the susceptible line T010000DH3. Genetic mapping was performed by identifying the percentage of polymorphic variants (PPV), a new method proposed in this study, through bulked segregant RNA sequencing. Chromosome C7 carried the highest PPV (42%) compared to the 30-34% in the remaining chromosomes. A peak with PPV (56-73%) was found within the physical interval 41-44 Mb, which indicated that Rcr7 might be located in this region. Kompetitive Allele-Specific PCR was used to confirm the association of Rcr7 with SNPs in the region. Rcr7 was flanked by two SNP markers and co-segregated with three SNP markers in the segregating population of 465 plants. Seven genes encoding TIR-NBS-LRR disease resistance proteins were identified in the target region, but only two genes, Bo7g108760 and Bo7g109000, were expressed. Resistance to pathotype 5X was also mapped to the same region as Rcr7. B. oleracea lines including 'Kilaherb' were tested with five SNP markers for Rcr7 and for resistance to pathotype 3; 11 of 25 lines were resistant, but 'Kilaherb' was the only line that carried the SNP alleles associated with Rcr7. The presence of Rcr7 in 'Kilaherb' for resistance to both pathotypes 3 and 5X was confirmed through linkage analysis.
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