ArticleProceedings of the National Academy of Sciences of the United States of America2018
VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Structure-guided optimization of SLY1 expression and purification in Escherichia coli.Protein science : a publication of the Protein Society · 2026Article
- Cloneable contrast across all biological length scales.Journal of structural biology · 2026Article
- The CRY1-HsF predicted interaction interface serves as a molecular platform for bioengineering or selecting modulating mutants.Frontiers in plant science · 2025Article
- Analytical Post-Embedding Immunogold-Electron Microscopy with Direct Gold-Labelled Monoclonal Primary Antibodies against RIBEYE A- and B-Domain Suggests a Refined Model of Synaptic Ribbon Assembly.International journal of molecular sciences · 2024Article
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins.Biochemistry · 2023Article
- Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane.Cell reports methods · 2022Article
- Heterogeneous protein co-assemblies with tunable functional domain stoichiometry.Molecular systems design & engineering · 2022Article
- Strategies for Site-Specific Labeling of Receptor Proteins on the Surfaces of Living Cells by Using Genetically Encoded Peptide Tags.Chembiochem : a European journal of chemical biology · 2021Review
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Perfluorocarbons in Chemical Biology.Chembiochem : a European journal of chemical biology · 2020Review
- EGFR Expression in HER2-Driven Breast Cancer Cells.International journal of molecular sciences · 2020Article
- Article
- A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells.Nature communications · 2020Article
- MiniVIPER Is a Peptide Tag for Imaging and Translocating Proteins in Cells.Biochemistry · 2020Article
- A PopZ-linked apical recruitment assay for studying protein-protein interactions in the bacterial cell envelope.Molecular microbiology · 2019Article
- Implementing VIPER for Imaging Cellular Proteins by Fluorescence Microscopy.Bio-protocol · 2019Article
- Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.Bio-protocol · 2019Article
- Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Bio-protocol · 2019Article
- VIPERACS applied materials & interfaces · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Many discoveries in cell biology rely on making specific proteins visible within their native cellular environment. There are various genetically encoded tags, such as fluorescent proteins, developed for fluorescence microscopy (FM). However, there are almost no genetically encoded tags that enable cellular proteins to be observed by both FM and electron microscopy (EM). Herein, we describe a technology for labeling proteins with diverse chemical reporters, including bright organic fluorophores for FM and electron-dense nanoparticles for EM. Our technology uses versatile interacting peptide (VIP) tags, a class of genetically encoded tag. We present VIPER, which consists of a coiled-coil heterodimer formed between the genetic tag, CoilE, and a probe-labeled peptide, CoilR. Using confocal FM, we demonstrate that VIPER can be used to highlight subcellular structures or to image receptor-mediated iron uptake. Additionally, we used VIPER to image the iron uptake machinery by correlative light and EM (CLEM). VIPER compared favorably with immunolabeling for imaging proteins by CLEM, and is an enabling technology for protein targets that cannot be immunolabeled. VIPER is a versatile peptide tag that can be used to label and track proteins with diverse chemical reporters observable by both FM and EM instrumentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.