ArticleBMC microbiology2018
Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia.
Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 43 citations in OpenAlex.
- Construction and Comparison of Molecular Identification Methods forMicroorganisms · 2026Article
- Molecular Detection and Antibiotic Susceptibility Profiling of Mannheimia haemolytica Isolates From Pneumonic Pasteurellosis Suspected Cases of Sheep in Northwestern Ethiopia.Veterinary medicine and science · 2026Article
- Multidimensional Phenotypic and Microbiome Studies Uncover an Association Between Reduced Feed Efficiency in Sheep During Mycoplasmal Pneumonia and Microbial Crosstalk Within the Rumen-Lung Axis.Veterinary sciences · 2025Article
- Single nucleotide polymorphisms, gene expression and evaluation of immunological, antioxidant, and pathological parameters associated with bacterial pneumonia in Barki sheep.Irish veterinary journal · 2025Article
- Genotyping and phylogenetic analysis ofVeterinary research forum : an international quarterly journal · 2025Article
- Evaluation of the effect of therapeutic durations on small ruminant bacterial pneumonia.BMC veterinary research · 2024Article
- Isolation and Identification ofTheScientificWorldJournal · 2024Article
- Identification of serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia.Scientific reports · 2023Article
- First study on capsular serotypes and virulence factors ofVeterinary world · 2023Article
- Isolation and Molecular Detection ofVeterinary medicine (Auckland, N.Z.) · 2023Article
- Serotyping, antibiogram, and detection of bacterial pathogens associated with bovine respiratory disease in selected areas of Ethiopia.Irish veterinary journal · 2022Article
- A Preliminary Investigation on a Commercial Ovine Pasteurellosis Vaccine Using Clinical and Pathological Endpoints.Infection and drug resistance · 2022Article
- Serotyping and Seroprevalence ofFrontiers in veterinary science · 2022Article
- Isolation and Molecular Detection of Pasteurellosis from Pneumonic Sheep in Selected Areas of Amhara Region, Ethiopia: An Implication for Designing Effective Ovine Pasteurellosis Vaccine.Veterinary medicine (Auckland, N.Z.) · 2022Article
- Biological and molecular characterization of a sheep pathogen isolate ofVeterinary world · 2021Article
- Genome Sequence ofMicrobiology resource announcements · 2021Article
- Serotyping, Genotyping and Virulence Genes Characterization ofVeterinary sciences · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMannheimia haemolytica has been recognized as the principal cause of pneumonic pasteurellosis in sheep and goats. It is one of the important diseases of small ruminants in Ethiopia. While annual vaccination using a monovalent vaccine (inactivated Pasteurella multocida biotype A) is common, respiratory diseases are still reported in various parts of Ethiopia. This suggests the need for further investigation into the species and strains responsible for the disease, which is vital information for development of a multivalent vaccine. The objective of the current study was to isolate M. heamolytica associated with pneumonic cases of sheep in selected areas of Central Ethiopia, determine its role and the strains/genotypes of the bacterium circulating in the study area.
resultsBacteriological analysis of nasal swab samples collected from a total of 76 pneumonic cases of sheep showed that M. haemolytica was isolated from 26 of them while B.trehalosi from two cases. Further molecular analyses of the isolates using M. haemolytica species-specific and M.haemolytica serotype-1 antigen specific PCR assays revealed, 26 of the isolates were identified as M. haemolytica of which 21 of them were M. haemolytica serotype-1. Both M. haemolytica and B.trehalosi isolates were not detected in a PCR assay targeting capsular biosynthesis gene (capA) of P.multocida despite the non-specific products observed in M. haemolytica isolates. Phylogenetic analysis of M. haemolytica isolates included in this study in comparison with the reference strains with respect to PHSSA and Rpt2 genes revealed that the Ethiopian M. haemolytica isolates constituted three distinct genotypes consistent with site of origin.
conclusionThe study indicated that M.haemolytica is commonly associated with cases of pneumonia in sheep in the study areas of central Ethiopia although the remaining other pathogens responsible for majority of the cases are yet to be determined. Molecular characterization revealed the existence of three genotypes of M. haemolytica circulating in the study areas consistent to the site of isolation. The findings suggest further extensive work to determine all pathogens associated with sheep pneumonia and the strain distribution of M. heamolytica to understand its molecular epidemiology at national level and design cost effective prevention and control methods.
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