ArticleCell reports2018
MadID, a Versatile Approach to Map Protein-DNA Interactions, Highlights Telomere-Nuclear Envelope Contact Sites in Human Cells.
Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Genome-wide chromatin recording resolves dynamic cell state changes.Cell systems · 2026Article
- Genome-wide chromatin recording resolves dynamic cell state changes.bioRxiv : the preprint server for biology · 2026Article
- A metabolism-chromatin axis promotes differential ribosomal RNA transcription in the human malaria parasite.Nature communications · 2025Article
- SUPREM: an engineered non-site-specific m6A RNA methyltransferase with highly improved efficiency.Nucleic acids research · 2024Article
- Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF.iScience · 2024Article
- Review
- Article
- DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome wide.Nature methods · 2022Article
- Epigenetic patterns in a complete human genome.Science (New York, N.Y.) · 2022Article
- Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing.Genome research · 2022Article
- Advances in enzyme-mediated proximity labeling and its potential for plant research.Plant physiology · 2022Article
- Nuclear Lamins: Key Proteins for Embryonic Development.Biology · 2022Review
- Increase in lamin B1 promotes telomere instability by disrupting the shelterin complex in human cells.Nucleic acids research · 2021Article
- Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP pools.Scientific reports · 2021Article
- Review
- Cell cycle dynamics of lamina-associated DNA.EMBO reports · 2020Article
- Dam mutants provide improved sensitivity and spatial resolution for profiling transcription factor binding.Epigenetics & chromatin · 2019Article
- DamID as a versatile tool for understanding gene regulation.Development (Cambridge, England) · 2019Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Mapping the binding sites of DNA- or chromatin-interacting proteins is essential to understanding biological processes. DNA adenine methyltransferase identification (DamID) has emerged as a comprehensive method to map genome-wide occupancy of proteins of interest. A caveat of DamID is the specificity of Dam methyltransferase for GATC motifs that are not homogenously distributed in the genome. Here, we developed an optimized method named MadID, using proximity labeling of DNA by the methyltransferase M.EcoGII. M.EcoGII mediates N6-adenosine methylation in any DNA sequence context, resulting in deeper and unbiased coverage of the genome. We demonstrate, using m6A-specific immunoprecipitation and deep sequencing, that MadID is a robust method to identify protein-DNA interactions at the whole-genome level. Using MadID, we revealed contact sites between human telomeres, repetitive sequences devoid of GATC sites, and the nuclear envelope. Overall, MadID opens the way to identification of binding sites in genomic regions that were largely inaccessible.
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Registered trials
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