ArticlePlant & cell physiology2019
Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm.
Article in Plant & cell physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.Rice (New York, N.Y.) · 2026Article
- Decoding heat-induced chalkiness in rice: molecular mechanisms, genetic networks, and mitigation strategies for climate resilience.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Article
- A molecular module improves rice grain quality and yield at high temperatures.National science review · 2025Article
- Natural variation in LONELY GUY-Like 1 regulates rice grain weight under warmer night conditions.Plant physiology · 2024Article
- The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma.Plant methods · 2024Article
- Microplastic Has No Effect on Rice Yield and Gaseous N Emission from an Infertile Soil with High Inorganic N Inputs.Plants (Basel, Switzerland) · 2024Article
- High Daytime Temperature Responsive MicroRNA Profiles in Developing Grains of Rice Varieties with Contrasting Chalkiness.International journal of molecular sciences · 2023Article
- HEAT SHOCK PROTEIN 90.6 interacts with carbon and nitrogen metabolism components during seed development.Plant physiology · 2023Article
- Molecular mapping and characterization of QTLs for grain quality traits in a RIL population of US rice under high nighttime temperature stress.Scientific reports · 2023Article
- Meta-QTL and haplo-pheno analysis reveal superior haplotype combinations associated with low grain chalkiness under high temperature in rice.Frontiers in plant science · 2023Article
- Organic Rice Production Practices: Effects on Grain End-Use Quality, Healthfulness, and Safety.Foods (Basel, Switzerland) · 2022Review
- DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate.Plant methods · 2022Article
- Amino Acids in Rice Grains and Their Regulation by Polyamines and Phytohormones.Plants (Basel, Switzerland) · 2022Review
- Laser microdissection transcriptome data derived gene regulatory networks of developing rice endosperm revealed tissue- and stage-specific regulators modulating starch metabolism.Plant molecular biology · 2022Article
- RiceFrontiers in plant science · 2022Article
- Substitution Mapping of Two Closely Linked QTLs on Chromosome 8 Controlling Grain Chalkiness in Rice.Rice (New York, N.Y.) · 2021Article
- Genome-wide association coupled gene to gene interaction studies unveil novel epistatic targets among major effect loci impacting rice grain chalkiness.Plant biotechnology journal · 2021Article
- Fine mapping of two grain chalkiness QTLs sensitive to high temperature in rice.Rice (New York, N.Y.) · 2021Article
- Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants.International journal of molecular sciences · 2020Review
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat stress occurrence during seed filling leads to the formation of a chalky portion in the limited zone of the starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene expression analysis of a 44 K microarray was performed to identify key regulatory genes involved in the formation of milky-white (MW) and white-back (WB) grains during heat stress. Gene regulatory network analysis classified the genes changed under heat stress into five modules. The most distinct expression pattern was observed in modules where most of the small heat shock proteins and cellular organization genes were changed under heat stress in dorsal aleurone cells and dorsal starchy endosperm zones. The histological observation supported the significant increase in cell number and size of dorsal aleurone cells in WB grains. With regard to the central starchy endosperm zone, preferential down-regulation of high molecular weight heat shock proteins (HMW HSPs), including a prominent member encoding endoplasmic reticulum (ER) chaperones, by heat stress was observed, while changes in expression of starch biosynthesis genes were minimal. Characterization of transgenic plants suppressing endosperm lumenal binding protein gene (BiP1), an ER chaperone preferentially down-regulated at the MW zone under heat stress, showed evidence of forming the chalky grains without disturbing the expression of starch biosynthesis genes. The present LM-based comprehensive expression analysis provides novel inferences that HMW HSPs play an important role in controlling redox, nitrogen and amino acid metabolism in endosperm leading to the formation of MW and WB chalky grains under heat stress.
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