ArticleJournal of experimental botany2019
A single serine to alanine substitution decreases bicarbonate affinity of phosphoenolpyruvate carboxylase in C4Flaveria trinervia.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 29 citations in OpenAlex.
- Novel Amino Acid Changes Increase Phosphoenolpyruvate Carboxylase Output in planta.Plant, cell & environment · 2026Article
- Fitting photosynthetic carbon dioxide and irradiance response curves for C4 leaves.Journal of experimental botany · 2026Article
- Environmental factors have a greater influence on photosynthetic capacity in CThe New phytologist · 2025Article
- Testing the kinetic tradeoff between bicarbonate versus phosphoenolpyruvate affinity and glucose-6 phosphate response of phosphoenolpyruvate carboxylase from two CPhotosynthesis research · 2025Article
- Leaf transcriptomes from C3, C3-C4 intermediate, and C4Neurachne species give insights into C4 photosynthesis evolution.Plant physiology · 2024Article
- Genomic and Transcriptomic Insights into the Evolution of C4 Photosynthesis in Grasses.Genome biology and evolution · 2024Article
- Multiple highly expressed phosphoenolpyruvate carboxylase genes have divergent enzyme kinetic properties in two C4 grasses.Annals of botany · 2023Article
- Detecting macroevolutionary genotype-phenotype associations using error-corrected rates of protein convergence.Nature ecology & evolution · 2023Article
- AnBio-protocol · 2021Article
- Expression of a COeLife · 2021Article
- Improving C4 photosynthesis to increase productivity under optimal and suboptimal conditions.Journal of experimental botany · 2021Article
- A low CO2-responsive mutant of Setaria viridis reveals that reduced carbonic anhydrase limits C4 photosynthesis.Journal of experimental botany · 2021Article
- Lateral Gene Transfer Acts As an Evolutionary Shortcut to Efficient C4 Biochemistry.Molecular biology and evolution · 2020Article
- Molecular Cloning of Novel-Type PhosphoPlants (Basel, Switzerland) · 2020Article
- Evolutionary Convergence of CFrontiers in plant science · 2020Article
- Kinetic Modifications of CFrontiers in plant science · 2020Article
- Techniques and challenges in studying photosynthetic diversity in freshwater submerged vascular plants.Applications in plant sciencesReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphoenolpyruvate (PEP) carboxylase (PEPc) catalyzes the first committed step of C4 photosynthesis generating oxaloacetate from bicarbonate (HCO3-) and PEP. It is hypothesized that PEPc affinity for HCO3- has undergone selective pressure for a lower KHCO3 (Km for HCO3-) to increase the carbon flux entering the C4 cycle, particularly during conditions that limit CO2 availability. However, the decrease in KHCO3 has been hypothesized to cause an unavoidable increase in KPEP (Km for PEP). Therefore, the amino acid residue S774 in the C4 enzyme, which has been shown to increase KPEP, should lead to a decrease in KHCO3. Several studies reported the effect S774 has on KPEP; however, the influence of this amino acid substitution on KHCO3 has not been tested. To test these hypotheses, membrane-inlet mass spectrometry (MIMS) was used to measure the KHCO3 of the photosynthetic PEPc from the C4Flaveria trinervia and the non-photosynthetic PEPc from the C3F. pringlei. The cDNAs for these enzymes were overexpressed and purified from the PEPc-less PCR1 Escherichia coli strain. Our work in comparison with previous reports suggests that KHCO3 and KPEP are linked by specific amino acids, such as S774; however, these kinetic parameters respond differently to the tested allosteric regulators, malate and glucose-6-phosphate.
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