ArticlePloS one2018
Development of a PCR algorithm to detect and characterize Neisseria meningitidis carriage isolates in the African meningitis belt.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Meningococcal carriage in UK university students post-pandemic: evidence for MenACWY vaccine impact and ongoing genogroup B risk.Frontiers in public health · 2026Article
- ModelingInfectious Disease Modelling · 2025Article
- Direct whole-genome sequencing enables strain typing of unculturableMicrobial genomics · 2025Article
- Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection ofCurrent issues in molecular biology · 2025Article
- Performance Comparison of Two In-House PCR Methods for DetectingDiagnostics (Basel, Switzerland) · 2025Article
- Article
- Development of a rapid, simple multiplex PCR-dipstick assay for the detection ofFrontiers in cellular and infection microbiology · 2025Article
- The genomic epidemiology ofThe Lancet regional health. Western Pacific · 2024Article
- Prevalence and persistence ofEpidemiology and infection · 2023Article
- Article
- Toward Establishing Integrated, Comprehensive, and Sustainable Meningitis Surveillance in Africa to Better Inform Vaccination Strategies.The Journal of infectious diseases · 2021Article
- Molecular diagnostic assays for the detection of common bacterial meningitis pathogens: A narrative review.EBioMedicine · 2021Review
- A Novel Platform Using RNA Signatures To Accelerate Antimicrobial Susceptibility Testing in Neisseria gonorrhoeae.Journal of clinical microbiology · 2020Article
- RNA markers for ultra-rapid molecular antimicrobial susceptibility testing in fluoroquinolone-treated Klebsiella pneumoniae.The Journal of antimicrobial chemotherapy · 2020Article
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Authors and funding
21 authors.
Funding
Abstract
Improved methods for the detection and characterization of carried Neisseria meningitidis isolates are needed. We evaluated a multiplex PCR algorithm for the detection of a variety of carriage strains in the meningitis belt. To further improve the sensitivity and specificity of the existing PCR assays, primers for gel-based PCR assays (sodC, H, Z) and primers/probe for real-time quantitative PCR (qPCR) assays (porA, cnl, sodC, H, E, Z) were modified or created using Primer Express software. Optimized multiplex PCR assays were tested on 247 well-characterised carriage isolates from six countries of the African meningitis belt. The PCR algorithm developed enabled the detection of N. meningitidis species using gel-based and real-time multiplex PCR targeting porA, sodC, cnl and characterization of capsule genes through sequential multiplex PCR assays for genogroups (A, W, X, then B, C, Y and finally H, E and Z). Targeting both porA and sodC genes together allowed the detection of meningococci with a sensitivity of 96% and 89% and a specificity of 78% and 67%, for qPCR and gel-based PCR respectively. The sensitivity and specificity ranges for capsular genogrouping of N. meningitidis are 67% - 100% and 98%-100% respectively for gel-based PCR and 90%-100% and 99%-100% for qPCR. We developed a PCR algorithm that allows simple, rapid and systematic detection and characterisation of most major and minor N. meningitidis capsular groups, including uncommon capsular groups (H, E, Z).
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