ArticleJournal of biomedical optics2018
Two-photon phosphorescence lifetime microscopy of retinal capillary plexus oxygenation in mice.
Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Quantifying retinal oxygenation and metabolism by phosphorescence lifetime imaging.Experimental eye research · 2025Review
- Multimodal retinal imaging by visible light optical coherence tomography and phosphorescence lifetime ophthalmoscopy in the mouse eye.Neurophotonics · 2025Article
- Confocal microscopic oxygen imaging of xenograft tumors using Ir(III) complexes as in vivo intravascular and intracellular probes.Scientific reports · 2024Article
- Versatile, in-line optical oxygen tension sensors for continuous monitoring duringSensors & diagnostics · 2024Article
- Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.Neurophotonics · 2023Article
- Biocompatible Phosphorescent OBiosensors · 2023Article
- Two-Photon Phosphorescence Lifetime Microscopy.Advances in experimental medicine and biology · 2021Article
- Three-Photon Spectroscopy of Porphyrins.The journal of physical chemistry. A · 2020Article
- In vivo OScientific reports · 2020Article
- Deep spectral learning for label-free optical imaging oximetry with uncertainty quantification.Light, science & applications · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Impaired oxygen delivery and/or consumption in the retinal tissue underlies the pathophysiology of many retinal diseases. However, the essential tools for measuring oxygen concentration in retinal capillaries and studying oxygen transport to retinal tissue are still lacking. We show that two-photon phosphorescence lifetime microscopy can be used to map absolute partial pressures of oxygen (pO2) in the retinal capillary plexus. Measurements were performed at various retinal depths in anesthetized mice under systemic normoxic and hyperoxic conditions. We used a newly developed two-photon phosphorescent oxygen probe, based on a two-photon absorbing platinum tetraphthalimidoporphyrin, and commercially available optics without correction for optical aberrations of the eye. The transverse and axial distances within the tissue volume were calibrated using a model of the eye's optical system. We believe this is the first demonstration of in vivo depth-resolved imaging of pO2 in retinal capillaries. Application of this method has the potential to advance our understanding of oxygen delivery on the microvascular scale and help elucidate mechanisms underlying various retinal diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.