ArticlePlant biotechnology journal2019
Wood forming tissue-specific bicistronic expression of PdGA20ox1 and PtrMYB221 improves both the quality and quantity of woody biomass production in a hybrid poplar.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Engineering 2-Pyrone-4,6-Dicarboxylic Acid Production Reveals Metabolic Plasticity of Poplar.Plant biotechnology journal · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- PtrVINV2 is dispensable for cellulose synthesis but essential for salt tolerance in Populus trichocarpa Torr. and Gray.Plant biotechnology journal · 2025Article
- CRISPR/Cas9-mediated disruption of DA1 enhances both biomass yield and quality in poplar.Plant biotechnology journal · 2025Article
- Novel synthetic inducible promoters controlling gene expression during water-deficit stress with green tissue specificity in transgenic poplar.Plant biotechnology journal · 2024Article
- Genomic and transcriptomic studies on flavonoid biosynthesis in Lagerstroemia indica.BMC plant biology · 2024Article
- PPGR: a comprehensive perennial plant genomes and regulation database.Nucleic acids research · 2024Article
- Exogenous gibberellin suppressed taproot secondary thickening by inhibiting the formation and maintenance of vascular cambium in radish (Frontiers in plant science · 2024Article
- Moderate Salinity Stress Increases the Seedling Biomass in Oilseed Rape (Plants (Basel, Switzerland) · 2023Article
- Overexpression of miR390b promotes stem elongation and height growth inHorticulture research · 2023Article
- Evidence for poplar PtaPLATZ18 in the regulation of plant growth and vascular tissues development.Frontiers in plant science · 2023Article
- Plant Development and Crop Yield: The Role of Gibberellins.Plants (Basel, Switzerland) · 2022Review
- Efficient knockout of the phytoene desaturase gene in a hybrid poplar (Populus alba × Populus glandulosa) using the CRISPR/Cas9 system with a single gRNA.Transgenic research · 2021Article
- CRISPR-Knockout ofInternational journal of molecular sciences · 2021Article
- Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue.Biotechnology for biofuels · 2021Article
- Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid Biosynthetic Pathways.International journal of molecular sciences · 2021Review
- Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees.Frontiers in plant science · 2021Review
- Genetic Modification ofFrontiers in plant science · 2021Article
- Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus.Scientific reports · 2020Article
- Overexpression of a Poplar RING-H2 Zinc Finger,International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the exponential growth of the human population and industrial developments, research on renewable energy resources is required to alleviate environmental and economic impacts caused by the consumption of fossil fuels. In this study, we present a synthetic biological application of a wood forming tissue-specific bicistronic gene expression system to improve both the quantity and quality of woody biomass to minimize undesirable growth penalties. Our transgenic poplars, designed to express both PdGA20ox1 (a GA20-oxidase from Pinus densiflora producing bioactive gibberellin, GA) and PtrMYB221 (a MYB transcription factor negatively regulating lignin biosynthesis) under the developing xylem (DX) tissue-specific promoter (i.e., DX15::PdGA20ox1-2A-PtrMYB221 poplar), resulted in a 2-fold increase in biomass quantity compared to wild-type (WT), without undesirable growth defects. A similar phenotype was observed in transgenic Arabidopsis plants harboring the same gene constructs. These phenotypic consequences were further verified in the field experiments. Importantly, our transgenic poplars exhibited an improved quality of biomass with reduced lignin content (~16.0 wt%) but increased holocellulose content (~6.6 wt%). Furthermore, the saccharification efficiency of our transgenic poplar increased significantly by up to 8%. Our results demonstrate that the controlled production of both GA and a secondary wall modifying regulator in the same spatio-temporal manner can be utilized as an efficient biotechnological tool for producing the desired multi-purpose woody biomass.
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