Evidence map›Paper›PMID 30514962›Full record

ArticleScientific reports2018

PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia.

Tatiana Lopatina, Enrica Favaro, Cristina Grange, Massimo Cedrino, Andrea Ranghino, Sergio Occhipinti, Sofia Fallo, Fabrizio Buffolo, Daria A Gaykalova, Maria M Zanone and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 3 countries.

Tatiana LopatinaDepartment of Medical Sciences, University of Turin, Turin, Italy. tatiana.lopatina@unito.it.
Enrica FavaroDepartment of Medical Sciences, University of Turin, Turin, Italy.
Cristina GrangeDepartment of Medical Sciences, University of Turin, Turin, Italy.
Massimo Cedrino2i3T, Società per La Gestione Dell'incubatore Di Imprese e Per Il Trasferimento Tecnologico Dell'Università degli Studi di Torino, Scarl., Molecular Biotechnology Center (MBC), Turin, Italy.
Andrea RanghinoDepartment of Medical Sciences, University of Turin, Turin, Italy.
Sergio OcchipintiDepartment of Medical Sciences, University of Turin, Turin, Italy.
Sofia FalloDepartment of Medical Sciences, University of Turin, Turin, Italy.
Fabrizio BuffoloDepartment of Medical Sciences, University of Turin, Turin, Italy.
Daria A GaykalovaOtolaryngology - Head and Neck Surgery, The Johns Hopkins University School of Medicine, 1550 Orleans street, Rm 5M06, CRBII, Baltimore, MD, 21231, United States of America.
Maria M ZanoneDepartment of Medical Sciences, University of Turin, Turin, Italy.
Renato RomagnoliGeneral Surgery 2U, Liver Transplantation Center, AOU Città della Salute e della Scienza di Torino, University of Turin, Turin, Italy.ORCID http://orcid.org/0000-0001-8340-8885
Giovanni CamussiDepartment of Medical Sciences, University of Turin, Turin, Italy.
University of Turin · ITDepartment of Medical Sciences · BYJohns Hopkins University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We previously have shown that platelet-derived growth factor (PDGF) modulates the biological activity of extracellular vesicles released by adipose-derived mesenchymal stem cells (ASC-EVs). ASC-EVs may interact with blood and vessel cells by transferring proteins and nucleic acids and regulate their functions. In this study, we investigated immunomodulatory activity and protection from acute hindlimb ischemia of EVs released by PDGF-stimulated ASC (PDGF-EVs). PDGF treatment of ASC changed protein and RNA composition of released EVs by enhancing the expression of anti-inflammatory and immunomodulatory factors. In vitro, control EVs (cEVs) derived from non-stimulated ASC increased the secretion of both the IL-1b, IL-17, IFNγ, TNFα pro-inflammatory factors and the IL-10 anti-inflammatory factor, and enhanced the in vitro peripheral blood mononuclear cell (PBMC) adhesion on endothelium. In contrast, PDGF-EVs enhanced IL-10 secretion and induced TGF-β1 secretion by PBMC. Moreover, PDGF-EVs stimulated the formation of T regulatory cells. In vivo, PDGF-EVs protected muscle tissue from acute ischemia, reduced infiltration of inflammatory cells and increased T regulatory cell infiltration in respect to cEVs. Our results suggest that PDGF-EVs are enriched in anti-inflammatory and immunomodulatory factors and induced in PBMC an enhanced production of IL-10 and TGF-β1 resulting in protection of muscle from acute ischemia in vivo.

Indexed as

AnimalsAnti-Inflammatory AgentsBiomarkersExtracellular VesiclesHindlimbImmunophenotypingIschemiaMesenchymal Stem CellsPeptidesPlatelet-Derived Growth FactorAnti-Inflammatory AgentsBiomarkersPeptidesPlatelet-Derived Growth Factor

Identifiers

PMID30514962
PMCPMC6279818
OpenAlexW2900456807

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.