ArticleEmerging microbes & infections2018
Novel type of pilus associated with a Shiga-toxigenic E. coli hybrid pathovar conveys aggregative adherence and bacterial virulence.
Article in Emerging microbes & infections, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 26 citations in OpenAlex.
- EnteroaggregativeEcoSal Plus · 2025Review
- Importance ofMicrobiology spectrum · 2025Article
- Molecular-based evidence for school transmission of enteroaggregative Escherichia coli among apparently healthy children attending nursery, infant, and primary schools in Madrid (Spain).European journal of pediatrics · 2025Article
- Molecular Characterisation of Escherichia coli Collected From an Urban River in Johannesburg, South Africa.Environmental microbiology reports · 2025Article
- Enteroaggregative Escherichia coli (EAEC) isolates obtained from non-diarrheic children carry virulence factor-encoding genes from Extraintestinal Pathogenic E. Coli (ExPEC).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Characterization of clumpy adhesion ofMicrobiology spectrum · 2024Article
- Article
- O-Antigen Diversification Masks Identification of Highly Pathogenic Shiga Toxin-Producing Escherichia coli O104:H4-Like Strains.Microbiology spectrum · 2023Article
- Transposon mutagenesis of atypical enteroaggregativeFrontiers in cellular and infection microbiology · 2023Article
- Role of aggregate-forming pilus (AFP) in adherence and colonization of both intestinal and urinary tracts.Virulence · 2022Article
- Adhesin related genes as potential markers for the enteroaggregativeFrontiers in cellular and infection microbiology · 2022Article
- The Superior Adherence Phenotype of E. coli O104:H4 is Directly Mediated by the Aggregative Adherence Fimbriae Type I.Virulence · 2021Article
- The aggregate-forming pili (AFP) mediates the aggregative adherence of a hybrid-pathogenicVirulence · 2021Article
- Bacteria autoaggregation: how and why bacteria stick together.Biochemical Society transactions · 2021Review
- AtaT Improves the Stability of Pore-Forming Protein EspB by Acetylating Lysine 206 to Enhance Strain Virulence.Frontiers in microbiology · 2021Article
- Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical EnteroaggregativeFrontiers in cellular and infection microbiology · 2020Article
- Diversity of Hybrid- and Hetero-PathogenicFrontiers in cellular and infection microbiology · 2020Review
- High variation acrossGut microbesArticle
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7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large German outbreak in 2011 was caused by a locus of enterocyte effacement (LEE)-negative enterohemorrhagic E. coli (EHEC) strain of the serotype O104:H4. This strain harbors markers that are characteristic of both EHEC and enteroaggregative E. coli (EAEC), including aggregative adhesion fimbriae (AAF) genes. Such rare EHEC/EAEC hybrids are highly pathogenic due to their possession of a combination of genes promoting severe toxicity and aggregative adhesion. We previously identified novel EHEC/EAEC hybrids and observed that one strain exhibited aggregative adherence but had no AAF genes. In this study, a genome sequence analysis showed that this strain belongs to the genoserotype O23:H8, MLST ST26, and harbors a 5.2 Mb chromosome and three plasmids. One plasmid carries some EAEC marker genes, such as aatA and genes with limited protein homology (11-61%) to those encoding the bundle-forming pilus (BFP) of enteropathogenic E. coli. Due to significant protein homology distance to known pili, we designated these as aggregate-forming pili (AFP)-encoding genes and the respective plasmid as pAFP. The afp operon was arranged similarly to the operon of BFP genes but contained an additional gene, afpA2, which is homologous to afpA. The deletion of the afp operon, afpA, or a nearby gene (afpR) encoding an AraC-like regulator, but not afpA2, led to a loss of pilin production, piliation, bacterial autoaggregation, and importantly, a >80% reduction in adhesion and cytotoxicity toward epithelial cells. Gene sets similar to the afp operon were identified in a variety of aatA-positive but AAF-negative intestinal pathogenic E. coli. In summary, we characterized widely distributed and novel fimbriae that are essential for aggregative adherence and cytotoxicity in a LEE-negative Shiga-toxigenic hybrid.
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