ArticleBMC veterinary research2018
Modulation of intestinal microbiota, morphology and mucin composition by dietary insect meal inclusion in free-range chickens.
Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Edible Insect Consumption for Human and Planetary Health: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Review
- Medium-chain fatty acid-rich oils with varying free fatty acid levels as alternatives to soybean oil in broiler starter diets.Poultry science · 2026Article
- Coffee Pulp and Silverskin Mitigate Fructose-Induced Intestinal Alterations in Rats.Biomolecules · 2026Article
- Nutritional alternatives to commercial lipid sources: Impact of the dietary inclusion of black soldier fly (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Enrichment with Hermetia illucens live larvae in a cage-free system: effects on welfare and egg production and quality in laying hens of two genotypes.Poultry science · 2026Article
- The chicken gut microbiome in conventional and alternative production systems.Journal of animal science and biotechnology · 2025Review
- The Effect of Oral ProbioticJournal of microbiology and biotechnology · 2025Article
- Growth rates and metabolic traits differ by diarrhoeal manifestation inJournal of medical microbiology · 2025Article
- Effects of live black soldier fly and yellow mealworm larvae supplementation on slaughter performance and meat composition of Muscovy ducks.Scientific reports · 2025Article
- Antioxidant Synergy in a Mixture of Powder Plant Leaves and Effects on Metabolic Profile, Oxidative Status and Intestinal Morpho-Histochemical Features of Laying Hens.Animals : an open access journal from MDPI · 2025Article
- Inclusion of Tenebrio molitor larvae meal in the diet of barbary partridge (Alectoris barbara) improves caecal bacterial diversity and composition.Scientific reports · 2024Article
- The effect of peanut skins as a natural antimicrobial feed additive on ileal and cecal microbiota in broiler chickens inoculated with Salmonella enterica Enteritidis.Poultry science · 2024Article
- Use ofInsects · 2024Article
- Insect live larvae as a new nutritional model in duck: effects on gut health.Animal microbiome · 2024Article
- Diet replacement with whole insect larvae affects intestinal morphology and microbiota of broiler chickens.Scientific reports · 2024Article
- Microbiota characterization throughout the digestive tract of horses fed a high-fiber vs. a high-starch diet.Frontiers in veterinary science · 2024Article
- Utilization of mannan oligosaccharides as antibiotic substitutes in laying hens.Veterinary research forum : an international quarterly journal · 2024Article
- A Systematic Review and Metanalysis on the Use ofVeterinary sciences · 2023Review
- Optimization of Xylooligosaccharides Production by Native and Recombinant Xylanase Hydrolysis of Chicken Feed Substrates.International journal of molecular sciences · 2023Article
10 more citing papers are in PubMed but not listed here.
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11 authors.
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Abstract
backgroundGut health in poultry depends on the balance between the host, intestinal microbiota, intestinal microscopic features and diet. The effects of insect meal (a promising alternative protein source for poultry feed) on chicken gut morphology have recently been reported, but no data about intestinal microbiota and mucin composition modulation are available. The present study evaluated the effects of dietary Tenebrio molitor (TM) meal inclusion on gut health of free-range chickens by intestinal microbiota, morphology and mucin composition characterization.
resultsOne hundred forty female medium-growing hybrids were divided into 2 dietary treatments (control feed [C] and 7.5% TM inclusion, with 5 replicate pens/treatment and 14 birds/pen) and slaughtered at 97 days of age (2 birds/pen for a total of 10 chickens/diet). The gut microbiota assessment on cecal content samples by 16S rRNA amplicon based sequencing showed higher alpha (Shannon, P < 0.05) and beta (Adonis and ANOSIM, P < 0.001) diversity in birds fed TM diet than C. In comparison with C group, TM birds displayed significant increase and decrease, respectively, of the relative abundances of Firmicutes and Bacteroidetes phyla, with higher Firmicutes:Bacteroidetes ratios (False Discovery Rate [FDR] < 0.05). The relative abundance of Clostridium, Oscillospira, Ruminococcus, Coprococcus and Sutterella genera was higher in TM chickens than C (FDR < 0.05). On the contrary, TM birds displayed significant decrease of the relative abundance of Bacteroides genus compared to the C group (FDR < 0.05). Gut morphology evaluation by morphometric analysis on small intestine revealed similar villus height, crypt depth and villus height to crypt depth ratio between C and TM birds. Characterization of gut mucin composition by periodic-acid Schiff, Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine showed unaffected mucin staining intensity in TM chickens when compared to C group.
conclusionsDietary TM meal inclusion may positively modulate the gut microbiota of the free-range chickens without influencing the intestinal morphology and mucin composition. Since the rapid growth of chickens directly depends on morphological and functional integrity of the digestive tract, the gut health assessment by a post mortem multidisciplinary approach appears to be fundamental.
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