Evidence map›Paper›PMID 30513899›Full record

ArticleInternational journal of molecular sciences2018

Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS.

Carsten Schmelter, Sebastian Funke, Jana Treml, Anja Beschnitt, Natarajan Perumal, Caroline Manicam, Norbert Pfeiffer, Franz H Grus

Open access · goldAbstract readComparative Study
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 42 citations in OpenAlex.

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  6. Bottom-Up Proteomics: Advancements in Sample Preparation.International journal of molecular sciences · 2023
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  10. Quantitative Proteomics ofPlants (Basel, Switzerland) · 2022
    Article
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  12. Mono-Sized Anion-Exchange Magnetic Microspheres for Protein Adsorption.International journal of molecular sciences · 2022
    Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Carsten SchmelterDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. cschmelter@eye-research.org.
Sebastian FunkeDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. sfunke@eye-research.org.
Jana TremlDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. jana-treml@gmx.de.
Anja BeschnittDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. a.beschnitt@gmx.net.ORCID 0000-0001-8949-4286
Natarajan PerumalDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. nperumal@eye-research.org.
Caroline ManicamDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. caroline.manicam@unimedizin-mainz.de.ORCID 0000-0001-5891-1155
Norbert PfeifferDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. norbert.pfeiffer@unimedizin-mainz.de.
Franz H GrusDepartment of Experimental and Translational Ophthalmology, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. grus@eye-research.org.
Johannes Gutenberg University Mainz · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proper sample preparation protocols represent a critical step for liquid chromatography-mass spectrometry (LC-MS)-based proteomic study designs and influence the speed, performance and automation of high-throughput data acquisition. The main objective of this study was to compare two commercial solid-phase extraction (SPE)-based sample preparation protocols (comprising SOLAµ

Indexed as

AnimalsBiomarkersChromatography, LiquidEye ProteinsGlaucomaPeptidesRetinaSolid Phase ExtractionSwineTandem Mass SpectrometryBiomarkersEye ProteinsPeptidesbiomarkersglaucoma animal modelmass spectrometrysample clean-upSOLAμTM HRP SPE spin platesZIPTIP® C18 pipette tips

Identifiers

PMID30513899
PMCPMC6321002
OpenAlexW2902010753

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.