Therapeutic Effect Enhancement by Dual-bias High-voltage Circuit of Transmit Amplifier for Immersion Ultrasound Transducer Applications.

The dual-bias high-voltage circuit of a transmit amplifier for immersion ultrasound transducer applications is proposed to enhance the therapeutic effect of human HeLa cells. Highvoltage output signals generated from a transmit amplifier are typically preferable for immersion ultrasound transducers owing to their high sensitivity at the desired frequency. However, highvoltage output signals typically produce high-order harmonic distortions, thus triggering several unwanted high-order spectral signals in the ultrasound transducers. By reducing high-order harmonic distortions, we expect that improving the signal quality of excited pulses for immersion ultrasound transducers would be beneficial for the therapeutic effect on human cervical cancer HeLa cell suppression. Therefore, an additional bias circuit is developed to merge with the original bias circuit for transmit amplifier to control the harmonic distortions of the immersion ultrasonic transducer. To properly select the components of dual-bias high-voltage circuit, we need to calculate and measure the DC bias voltages of the transmit amplifier with and without dual-bias high-voltage circuit for different period of the time for therapeutic applications. To evaluate the performances of the developed circuit, pulse-echo measurements using a transmit amplifier with or without dualbias high-voltage circuit were obtained. The measured second, third, and fourth harmonic distortions of the echo signals when using the transmit amplifier with dual-bias high-voltage circuit at 10 V DC bias voltage are lower than those when using the transmit amplifier only. Subsequently, the therapeutic effects using the enhanced performances of the transmit amplifier with dual-bias high-voltage circuit were verified and compared with those using the performances of the transmit amplifier by comparison of quantitative changes in HeLa cell concentrations. The control group without any ultrasonic induction increased the cell density up to about 100% on Day4, however the experimental groups with ultrasonic induction (TA = 91.2 ± 0.8%, TA+Dual-bias high-voltage circuit (0.8V) = 78.8±1.7% and TA+Dual-bias high-voltage circuit (10 V) = 66.3 ± 1.1%) showed statistically significant cell density changes compared to the control group. We confirmed that the therapeutic effect from using the dual-bias high-voltage circuit is improved. Therefore, it can be a potential candidate to improve the therapeutic effect of HeLa cells.