ArticleBioconjugate chemistry2019
Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds.
Article in Bioconjugate chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- 3D Printable Poly(ACS applied polymer materials · 2024Review
- Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Gelatin methacryloyl granular scaffolds for localized mRNA delivery.Aggregate (Hoboken, N.J.) · 2024Article
- The application and prospects of 3D printable microgel in biomedical science and engineering.International journal of bioprinting · 2023Review
- Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.Journal of visualized experiments : JoVE · 2022Article
- Sticking Together: Injectable Granular Hydrogels with Increased Functionality via Dynamic Covalent Inter-Particle Crosslinking.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke.Advanced therapeutics · 2022Article
- Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications.Journal of visualized experiments : JoVE · 2022Article
- Mechanical reinforcement of granular hydrogels.Chemical science · 2022Review
- Nucleic Acid Delivery from Granular Hydrogels.Advanced healthcare materials · 2022Article
- Influence of Microgel Fabrication Technique on Granular Hydrogel Properties.ACS biomaterials science & engineering · 2021Article
- Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems.Journal, genetic engineering & biotechnology · 2021Review
- Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments.Advanced functional materials · 2020Article
- Click by Click Microporous Annealed Particle (MAP) Scaffolds.Advanced healthcare materials · 2020Article
- Hydrogel microparticles for biomedical applications.Nature reviews. Materials · 2020Article
- Bio-orthogonal, Site-Selective Conjugation of Recombinant Proteins to Microporous Annealed Particle Hydrogels for Tissue Engineering.Advanced therapeutics · 2020Article
- Targeted Drug Delivery via the Use of ECM-Mimetic Materials.Frontiers in bioengineering and biotechnology · 2020Review
- Injectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery.Cellular and molecular bioengineering · 2019Article
- Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer.Acta biomaterialia · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gene delivery using injectable hydrogels can serve as a potential method for regulated tissue regeneration in wound healing. Our microporous annealed particle (MAP) hydrogel has been shown to promote cellular infiltration in both skin and brain wounds, while reducing inflammation. Although the scaffold itself can promote healing, it is likely that other signals will be required to promote healing of hard-to-treat wounds. Gene delivery is one approach to introduce desired bioactive signals. In this study, we investigated how the properties of MAP hydrogels influence non-viral gene delivery of polyethylenimine-condensed plasmid to cells seeded within the MAP gel. From past studies, we found that gene transfer to cells seeded in tissue culture plastic differed from gene transfer to cells seeded inside hydrogel scaffolds. Since MAP scaffolds are generated from hydrogel microparticles that are approximately 100 μm in diameter, they display local characteristics that can be viewed as two-dimensional or three-dimensional to cells. Thus, we sought to study if gene transfer inside MAP scaffolds differed from gene transfer to cells seeded in tissue culture plastic. We sought to understand the roles of the endocytosis pathway, actin and microtubule dynamics, RhoGTPases, and YAP/TAZ on transfection of human fibroblasts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.