Evidence map›Paper›PMID 30511674›Full record

ArticleActa crystallographica. Section F, Structural biology communications2018

An inexpensive system for imaging the contents of multi-well plates.

Andrew Bohm

Abstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Journal of applied crystallography · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Acta crystallographica. Section F, Structural biology communications · 2019
    Article
  6. AMi: a GUI-based, open-source system for imaging samples in multi-well plates.Acta crystallographica. Section F, Structural biology communications · 2019
    Article
  7. Introducing Methods Communications, a new category of contributions to Acta Crystallographica F.Acta crystallographica. Section F, Structural biology communications · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Andrew BohmDepartment of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

Funding

A structure-based approach to accelerated recombinase engineeringR01AI150499 · NIAID · TUFTS UNIVERSITY BOSTON · PI BOHM, ALEX ANDREW · 2020 to 2021
$495k
A structure-based approach to accelerated recombinase engineeringR01GM126149 · NIGMS · TUFTS UNIVERSITY BOSTON · PI BOHM, ALEX ANDREW · 2018 to 2019
$495k
NIAID NIH HHS R01 AI150499NIGMS NIH HHS R01 GM126149
6 · The paper itself

Abstract

An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or movies though a microscope-mounted digital camera. The translation stage provides reproducible three-dimensional movement of the sample over a volume of 160 mm in x, 100 mm in y and 40 mm in z. A Python script generates the G-code command file that scans the plate and collects a series of z-stacked images of each sample. A second Python script automates the calculation of images with a digitally enhanced depth of field. The imaging system is currently being used to facilitate screening for protein crystals, but it could be used to automate the imaging of many other types of samples in multi-well plates.

Indexed as

Cost-Benefit AnalysisImaging, Three-DimensionalMicroscopyautomated microscopeimaging systemmulti-well plates

Identifiers

PMID30511674
PMCPMC6277959

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.