ArticleScientific reports2018
Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Epididymis-specific RNase A family genes regulate fertility and small RNA processing.The Journal of biological chemistry · 2024Article
- Epididymis-specific RNase A family genes regulate fertility and small RNA processing.bioRxiv : the preprint server for biology · 2024Article
- A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability.Scientific reports · 2024Article
- Unlocking Genetic Mysteries during the Epic Sperm Journey toward Fertilization: Further ExpandingBiomolecules · 2024Review
- Distinct actions of testicular endocrine and lumicrine signaling on the proximal epididymal transcriptome.Reproductive biology and endocrinology : RB&E · 2024Article
- Bringing proteomics to bear on male fertility: key lessons.Expert review of proteomics · 2024Review
- Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation.International journal of molecular sciences · 2024Article
- Regulatory T cells play a crucial role in maintaining sperm tolerance and male fertility.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Various gene modification techniques to discover molecular targets for nonhormonal male contraceptives: A review.International journal of reproductive biomedicine · 2023Review
- The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†.Biology of reproduction · 2022Article
- Plasmodium falciparum Cysteine Rich Secretory Protein uniquely localizes to one end of male gametes.Molecular and biochemical parasitology · 2022Article
- Article
- Differential Effects of Low and High Radiation Dose Rates on Mouse Spermatogenesis.International journal of molecular sciences · 2021Article
- Activation of C-C motif chemokine receptor 2 modulates testicular macrophages number, steroidogenesis, and spermatogenesis progression.Cell and tissue research · 2021Article
- Origin and Evolution of the Gene Family of Proteinaceous Pheromones, the Exocrine Gland-Secreting Peptides, in Rodents.Molecular biology and evolution · 2021Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- Cysteine-Rich Secretory Proteins (CRISP) are Key Players in Mammalian Fertilization and Fertility.Frontiers in cell and developmental biology · 2021Review
- Pharmacological Inactivation of CatSper Blocks Sperm Fertilizing Ability Independently of the Capacitation Status of the Cells: Implications for Non-hormonal Contraception.Frontiers in cell and developmental biology · 2021Article
- Evolutionary analysis of genes coding for Cysteine-RIch Secretory Proteins (CRISPs) in mammals.BMC evolutionary biology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Epididymal Cysteine Rich Secretory Proteins 1 and 4 (CRISP1 and CRISP4) associate with sperm during maturation and play different roles in fertilization. However, males lacking each of these molecules individually are fertile, suggesting compensatory mechanisms between these homologous proteins. Based on this, in the present work, we generated double CRISP1/CRISP4 knockout (DKO) mice and examined their reproductive phenotype. Our data showed that the simultaneous lack of the two epididymal proteins results in clear fertility defects. Interestingly, whereas most of the animals exhibited specific sperm fertilizing ability defects supportive of the role of CRISP proteins in fertilization, one third of the males showed an unexpected epididymo-orchitis phenotype with altered levels of inflammatory molecules and non-viable sperm in the epididymis. Further analysis showed that DKO mice exhibited an immature epididymal epithelium and abnormal luminal pH, supporting these defects as likely responsible for the different phenotypes observed. These observations reveal that CRISP proteins are relevant for epididymal epithelium differentiation and male fertility, contributing to a better understanding of the fine-tuning mechanisms underlying sperm maturation and immunotolerance in the epididymis with clear implications for human epididymal physiology and pathology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.