Evidence map›Paper›PMID 30508719›Full record

ArticleiScience2018

Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression.

Rajendra K Gangalum, Dongjae Kim, Raj K Kashyap, Serghei Mangul, Xinkai Zhou, David Elashoff, Suraj P Bhat

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Rajendra K GangalumStein Eye Institute, Geffen School of Medicine, University of California, Los Angeles, CA 90095-7000, USA.
Dongjae KimStein Eye Institute, Geffen School of Medicine, University of California, Los Angeles, CA 90095-7000, USA.
Raj K KashyapStein Eye Institute, Geffen School of Medicine, University of California, Los Angeles, CA 90095-7000, USA.
Serghei MangulDepartment of Computer Science and Human Genetics, University of California, Los Angeles, CA 90095-7000, USA.
Xinkai ZhouDepartment of Medicine, University of California, Los Angeles, CA 90095-7000, USA.
David ElashoffDepartment of Medicine, University of California, Los Angeles, CA 90095-7000, USA.
Suraj P BhatStein Eye Institute, Geffen School of Medicine, University of California, Los Angeles, CA 90095-7000, USA; Brain Research Institute, University of California, Los Angeles, CA 90095-7000, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095-7000, USA. Electronic address: sbhat@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Childhood Cataractogenesis: Heterogeneity of Gene ExpressionR01EY024929 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BHAT, SURAJ P · 2015 to 2018
$1.6M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY024929
6 · The paper itself

Abstract

The developing eye lens presents an exceptional paradigm for spatial transcriptomics. It is composed of highly organized long, slender transparent fiber cells, which differentiate from the edges of the anterior epithelium of the lens (equator), attended by high expression of crystallins, which generates transparency. Every fiber cell, therefore, is an optical unit whose refractive properties derive from its gene activity. Here, we probe this tangible relationship between the gene activity and the phenotype by studying the expression of all known 17 crystallins and 77 other non-crystallin genes in single fiber cells isolated from three states/regions of differentiation, allowing us to follow molecular progression at the single-cell level. The data demonstrate highly variable gene activity in cortical fibers, interposed between the nascent and the terminally differentiated fiber cell transcription. These data suggest that the so-called stochastic, highly heterogeneous gene activity is a regulated intermediate in the realization of a functional phenotype.

Indexed as

Biological Sciences Research MethodologiesCell BiologyDevelopmental BiologyOmics

Identifiers

PMID30508719
PMCPMC6277220
OpenAlexW2900744787

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.