ArticleCell journal2019
Detection of Mycoplasma Contamination of Cell Culture by A Loop-Mediated Isothermal Amplification Method.
Article in Cell journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Simple, specific, rapid, and pharmacopoeia-compliant qPCR approach for the detection of mycoplasma in biopharmaceuticals.Molecular therapy. Methods & clinical development · 2025Article
- Comparative genomic analysis of Mycoplasma related to cell culture for infB gene-based loop-mediated isothermal amplification.World journal of microbiology & biotechnology · 2023Article
- Development and Evaluation of a New qPCR Assay for the Detection ofCurrent issues in molecular biology · 2023Article
- A hitchhiker's guide to cancer models.Trends in biotechnology · 2022Review
- Development of New PCR Assay with SYBR Green I for Detection ofDiagnostics (Basel, Switzerland) · 2021Article
- Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture.International journal of molecular sciences · 2020Article
- Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures.BMC research notes · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveMycoplasmas are major contaminants of cell culture and affect in vitro biological and diagnostic tests. Mycoplasma detection is conducted using culture and molecular methods. These methods vary in terms of accuracy, reliably and sensitivity. Loop-mediated isothermal amplification (LAMP) is used to amplify target DNA in a highly specific and rapid manner. This study aimed to develop a LAMP method for rapid detection of Mycoplasma in culture samples. MATERIALS AND
methodsIn this descriptive laboratory study, for LAMP detection of Mycoplasma contaminations in cell culture, we used primers specifically designed for targeting the 16S rRNA conserved gene of Mycoplasma spp. For a positive control structure, 16S rRNA amplified based on PCR, was cloned in a plasmid vector and sequenced. The assay specificity was evaluated using Mycoplasma genomic DNA and a panel containing genomes of gram-positive and gram-negative organisms.
resultsIn this study, the method developed for detection of Mycoplasma contamination of cell cultures was a rapid, sensitive and cost-effective LAMP approach. The results demonstrated that this method benefits from high specificity (100%) for amplification of Mycoplasma strains and high speed (multiplication within 60 minutes), while it does not require expensive laboratory equipment compared to those needed for polymerase chain reaction (PCR)-based detection.
conclusionOur study is the first report about application of LAMP assay based on 16S rRNA gene for detection of Mycoplasma strains; this technique could be considered a useful tool for rapid detection of contamination of cell culture.
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