ArticleSynthetic and systems biotechnology2019
Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells.
Article in Synthetic and systems biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- A portable Cas6f-based system for multiplex translational repression in bacteria.Nature communications · 2026Article
- Cross-Strain Transferability of CRISPRi Systems and Design Rules from Laboratory to ClinicalACS synthetic biology · 2026Article
- Cross-strain transferability of CRISPRi systems and design rules from laboratory to clinicalbioRxiv : the preprint server for biology · 2026Article
- Modular and signal-responsive transcriptional regulation using CRISPRi-aided genetic switches in Escherichia coli.Journal of biological engineering · 2025Article
- The rise and future of CRISPR-based approaches for high-throughput genomics.FEMS microbiology reviews · 2024Review
- Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.Nucleic acids research · 2024Article
- CRISPR-based screening of small RNA modulators of bile susceptibility inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genome-scale CRISPRi screening: A powerful tool in engineering microbiology.Engineering microbiology · 2023Review
- When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.Biophysical reviews · 2023Review
- Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes.AMB Express · 2023Article
- CRISPR-Based Approaches for Gene Regulation in Non-Model Bacteria.Frontiers in genome editing · 2022Review
- Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing inSynthetic and systems biotechnology · 2021Article
- CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application.Life (Basel, Switzerland) · 2021Review
- Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a.Nucleic acids research · 2021Article
- Recent advances of Cas12a applications in bacteria.Applied microbiology and biotechnology · 2021Review
- CRISPR-based gene expression control for synthetic gene circuits.Biochemical Society transactions · 2020Review
- Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas.Nucleic acids research · 2020Article
- CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference.Nucleic acids research · 2020Article
- CRISPR Tools To Control Gene Expression in Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Development and Application of CRISPR/Cas in Microbial Biotechnology.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With a unique crRNA processing capability, the CRISPR associated Cpf1 protein holds great potential for multiplex gene regulation. Unlike the well-studied Cas9 protein, however, conversion of Cpf1 to a transcription regulator and its related properties have not been systematically explored yet. In this study, we investigated the mutation schemes and crRNA requirements for the DNase deactivated Cpf1 (dCpf1). By shortening the direct repeat sequence, we obtained genetically stable crRNA co-transcripts and improved gene repression with multiplex targeting. A screen of diversity-enriched PAM library was designed to investigate the PAM-dependency of gene regulation by dCpf1 from
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.