Evidence map›Paper›PMID 30505943›Full record

ArticlemSystems

Qualitative and Quantitative DNA- and RNA-Based Analysis of the Bacterial Stomach Microbiota in Humans, Mice, and Gerbils.

Philipp Wurm, Elisabeth Dörner, Christina Kremer, Julia Spranger, Cynthia Maddox, Bettina Halwachs, Ute Harrison, Thomas Blanchard, Rainer Haas, Christoph Högenauer and 2 more

Open access · goldAbstract read
In one paragraph

Article in mSystems. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Re-framing the importance of Group BInfection and immunity · 2024
    Review
  6. Article
  7. The gut microbiota and its biogeography.Nature reviews. Microbiology · 2024
    Review
  8. Article
  9. Clinical Pathogenesis, Molecular Mechanisms of Gastric Cancer Development.Current topics in microbiology and immunology · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Philipp WurmInstitute of Pathology, Medical University Graz, Graz, Austria.
Elisabeth DörnerUniversität Hohenheim, Stuttgart, Germany.
Christina KremerUniversität Hohenheim, Stuttgart, Germany.
Julia SprangerUniversität Hohenheim, Stuttgart, Germany.
Cynthia MaddoxUniversität Hohenheim, Stuttgart, Germany.
Bettina HalwachsInstitute of Pathology, Medical University Graz, Graz, Austria.
Ute HarrisonMax von Pettenkofer-Institut, Ludwig-Maximilians-Universität München, Munich, Germany.
Thomas BlanchardDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
Rainer HaasMax von Pettenkofer-Institut, Ludwig-Maximilians-Universität München, Munich, Germany.
Christoph HögenauerTheodor Escherich Laboratory for Medical Microbiome Research, Medical University of Graz, Graz, Austria.
Gregor Gorkiewicz *Institute of Pathology, Medical University Graz, Graz, Austria.
W Florian Fricke *Universität Hohenheim, Stuttgart, Germany.ORCID https://orcid.org/0000-0003-1586-0962
University of Hohenheim · DEMedical University of Graz · ATLudwig-Maximilians-Universität München · DEUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clinical interventions in the stomach have been linked to fecal microbiota alterations, suggesting a function of the stomach in gastrointestinal (GI) homeostasis. We sought to determine the taxonomic bacterial biogeography of the upper GI tract, including different sites within the human stomach (cardia, corpus, and antrum), adjacent upstream (esophagus) and downstream (duodenum) locations, and luminal contents (aspirate), as well as whole-stomach samples from mice and gerbils. Qualitative and quantitative DNA- and RNA-based taxonomic microbiota analyses were combined to study the relationship of relative and absolute bacterial abundances and transcriptionally active bacterial microbiota components in the stomach of humans and mice. Stomach microbiota compositions resembled those of esophagus and duodenum. However, along the descending GI tract, the relative abundances of specific oropharyngeal commensals decreased (

Indexed as

16S rRNAabsolute abundancequantitative microbiota analysisstomach microbiotatranscriptional activity

Identifiers

PMID30505943
PMCPMC6247015
OpenAlexW2900466639

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.