Evidence map›Paper›PMID 30505885›Full record

ArticleBio-protocol2018

Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells.

Maria Daniela Garcia-Castillo, Wayne I Lencer, Daniel J-F Chinnapen

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Maria Daniela Garcia-CastilloDivision of Gastroenterology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Wayne I LencerDivision of Gastroenterology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Daniel J-F ChinnapenDivision of Gastroenterology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Harvard University · US

Funding

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ALIMENTARY TRACTP30DK034854 · NIDDK · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI JONATHAN C KAGAN · 1986 to 2026
$32.4M
Mechanism of action for the epithelial-specific ER stress sensor IRE1β in regulating intestinal homeostasis and host defenseR01DK048106 · NIDDK · CHILDREN'S HOSPITAL BOSTON · PI WAYNE I LENCER · 1993 to 2026
$9.6M
Intestinal Disease-enterocyte toxin interactionR37DK048106 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI LENCER, WAYNE I · 2011 to 2020
$7.0M
FcRn-dependent sorting of IgG and IgG-opsinized antigens by epithelial cellsR01DK084424 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI LENCER, WAYNE I · 2009 to 2013
$1.9M
Harnessing the biology of glycosphingolipid trafficking for biologic drug deliveryR01DK104868 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI LENCER, WAYNE I · 2016 to 2019
$1.8M
Development of a lipid-based platform for mucosal delivery and mucosal vaccinationF32DK111072 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI GARCIA-CASTILLO, MARIA DANIELA · 2016 to 2018
$131k
NIDDK NIH HHS F32 DK111072NIDDK NIH HHS P30 DK034854NIDDK NIH HHS R01 DK048106NIDDK NIH HHS R01 DK084424NIDDK NIH HHS R01 DK104868NIDDK NIH HHS R37 DK048106
6 · The paper itself

Abstract

Absorption and secretion of peptide and protein cargoes across single-cell thick mucosal and endothelial barriers occurs by active endocytic and vesicular trafficking that connects one side of the epithelial or endothelial cell (the lumen) with the other (the serosa or blood). Assays that assess this pathway must robustly control for non-specific and passive solute flux through weak or damaged intercellular junctions that seal the epithelial or endothelial cells together. Here we describe an

Indexed as

Drug deliveryEndocytic sortingEpithelial barriersGlycosphingolipidsTranscytosis

Identifiers

PMID30505885
PMCPMC6261372
OpenAlexW2896920648

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.