ArticleJournal of intensive care2018
Circulating histone H3 levels are increased in septic mice in a neutrophil-dependent manner: preclinical evaluation of a novel sandwich ELISA for histone H3.
Article in Journal of intensive care, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Proteomic identification of resistin as a NET-associated neutrophil activity marker in sepsis.Journal of intensive care · 2026Article
- Epigenetic regulation of neutrophil dysfunction in sepsis: mechanisms, biomarkers, and translational challenges.Journal of translational medicine · 2026Review
- Peritoneal neutrophil extracellular traps contribute to septic AKI via peritoneal IL-17A and distant organ CXCL-1/ CXCL-2 pathway in abdominal sepsis.Scientific reports · 2026Article
- Portable histone assay technology (PHAST) for the sample-to-answer detection of circulating histones in whole blood.Sensors and actuators. B, Chemical · 2026Article
- Peritoneal Neutrophil Extracellular Traps contribute to septic AKI via peritoneal IL-17A and distant organ CXCL-1/ CXCL-2 pathway in abdominal sepsis.Research square · 2025Article
- Effects of recombinant human soluble thrombomodulin on neutrophil extracellular traps in the kidney of a mouse model of endotoxin shock.Fujita medical journal · 2023Article
- Validation of circulating histone detection by mass spectrometry for early diagnosis, prognosis, and management of critically ill septic patients.Journal of translational medicine · 2023Article
- Association of circulating histone H3 and high mobility group box 1 levels with postoperative prognostic indicators in intensive care unit patients: a single-center observational study.Fujita medical journal · 2023Article
- Investigation of biomarkers in a rare case of fulminant necrotizing enterocolitis in a preterm infant.Fukushima journal of medical science · 2023Article
- Extra-nuclear histones: origin, significance and perspectives.Molecular and cellular biochemistry · 2022Review
- Histones: The critical players in innate immunity.Frontiers in immunology · 2022Review
- Association of Histones With Coagulofibrinolytic Responses and Organ Dysfunction in Adult Post-cardiac Arrest Syndrome.Frontiers in cardiovascular medicine · 2022Article
- Review
- Increased plasma levels of damage-associated molecular patterns during systemic anticancer therapy in patients with advanced lung cancer.Translational lung cancer research · 2021Article
- Release mechanisms of major DAMPs.Apoptosis : an international journal on programmed cell death · 2021Review
- Differential Regulation of Damage-Associated Molecular Pattern Release in a Mouse Model of Skeletal Muscle Ischemia/Reperfusion Injury.Frontiers in immunology · 2021Article
- Thromboplasminflammation in COVID-19 Coagulopathy: Three Viewpoints for Diagnostic and Therapeutic Strategies.Frontiers in immunology · 2021Review
- Circulating Histones in Sepsis: Potential Outcome Predictors and Therapeutic Targets.Frontiers in immunology · 2021Review
- Circulating intranuclear proteins may play a role in development of disseminated intravascular coagulation in individuals with acute leukemia.International journal of hematology · 2020Article
- Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNuclear histone proteins are released into the extracellular space during sepsis and act as major mediators of death. However, circulating histone levels have not been precisely quantified.
methodsWe developed a novel enzyme-linked immunosorbent assay (ELISA) for detection of circulating histone H3 levels and evaluated its performance. Using the ELISA, we measured plasma histone H3 levels in C57BL/6 J mice subjected to cecal ligation and puncture (CLP)-induced sepsis.
resultsThe newly developed ELISA enabled reproducible measurement of histone H3 levels with a working range up to 250 ng/mL. Using the ELISA, we found that plasma histone H3 levels were elevated in septic mice compared with sham-operated mice (
conclusionsOur novel ELISA provides reproducible measurements of histone H3 levels. Circulating histone H3 levels are increased in septic mice in a neutrophil-dependent manner. Further studies are needed to evaluate the clinical utility of histone H3 levels in patients with sepsis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.