Evidence map›Paper›PMID 30505333›Full record

ArticleEntomological research2018

Optimization of double-stranded RNAi intrathoracic injection method in

Seokyoung Kang, Dongyoung Shin, Mi Young Noh, Jill S Peters, Chelsea T Smartt, Yeon Soo Han, Young S Hong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Entomological research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Seokyoung KangDept. of Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
Dongyoung ShinDept. of Nematology and Entomology, University of Florida, Vero Beach, FL32962, USA.
Mi Young NohDept. of Agricultural Biology, Chonnam National University, Gwang-ju, 500-757, Republic of Korea.
Jill S PetersDept. of Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
Chelsea T SmarttDept. of Nematology and Entomology, University of Florida, Vero Beach, FL32962, USA.
Yeon Soo HanDept. of Agricultural Biology, Chonnam National University, Gwang-ju, 500-757, Republic of Korea.
Young S HongAccess Bio, Inc. Somerset, NJ 08873, USA.
Chonnam National University · KRTulane University · USUniversity of Florida · USSomerset Medical Center · US

Funding

Genomics of Antiviral Responses of Anopheles gambiae Against O'nyong-nyong VirusR21AI076774 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI HONG, YOUNG S · 2009 to 2010
$284k
NIAID NIH HHS R21 AI076774
6 · The paper itself

Abstract

RNA interference is widely used to analyze gene functions via phenotypic knockdown of target transcripts in mosquitoes, which transmit numerous mosquito-borne diseases. Functional analysis of mosquito genes is indispensable to understand and reduce transmission of mosquito-borne diseases in mosquitoes. Intrathoracic injection of double-stranded RNA (dsRNA) remains the simplest and most customizable method in mosquitoes for functional analysis of the genes of interest. However, achieving consistent and effective knockdown by dsRNAi is often elusive and may require extensive optimization. We tested the effectiveness of gene silencing by intrathoracic injection of four different quantities of dsRNA targeting two

Indexed as

dsRNA interference (dsRNAi)gene silencingintrathoracic injectionmosquito

Identifiers

PMID30505333
PMCPMC6261502
OpenAlexW2884511636

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.