ArticleApplied and environmental microbiology2019
Changes in the Rumen Microbiota of Cows in Response to Dietary Supplementation with Nitrate, Linseed, and Saponin Alone or in Combination.
Article in Applied and environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Influence of saponin extracts on enteric methane emission and rumen fermentation: a meta-analysis of in vitro experiments.BMC veterinary research · 2026Pooled it
- Calcium Nitrate Supplementation Improves Meat Quality in Hu Sheep via Microbial and Transcriptomic Regulation.Animals : an open access journal from MDPI · 2026Article
- Effects of dietary supplementation with linseed oil, Ascophyllum nodosum or treated A. nodosum on animal performance, gaseous emissions, ruminal fermentation and microbiota, and meat quality in growing dairy-beef bulls.Journal of animal science · 2025Article
- Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures.Life (Basel, Switzerland) · 2024Article
- Review
- Exploring the Bacterial Community in Aged Fecal Sources from Dairy Cows: Impacts on Fecal Source Tracking.Microorganisms · 2023Article
- The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows.Microbiome · 2022Article
- First Steps into Ruminal Microbiota Robustness.Animals : an open access journal from MDPI · 2022Review
- Effect of Hydrogen-Consuming Compounds on In Vitro Ruminal Fermentation, Fatty Acids Profile, and Microbial Community in Water Buffalo.Current microbiology · 2022Article
- Feeding Calcium-Ammonium Nitrate to Lactating Dairy Goats: Milk Quality and Ruminal Fermentation Responses.Animals : an open access journal from MDPI · 2022Article
- Stool and Ruminal Microbiome Components Associated With Methane Emission and Feed Efficiency in Nelore Beef Cattle.Frontiers in genetics · 2022Article
- Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Early life dietary intervention in dairy calves results in a long-term reduction in methane emissions.Scientific reports · 2021Article
- Shrub coverage alters the rumen bacterial community of yaks (Journal of animal science and technology · 2020Article
- Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress ConditionsArchaea (Vancouver, B.C.) · 2020Article
- Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet-Microbe Interaction.Frontiers in veterinary science · 2020Review
- Impacts of Mootral on Methane Production, Rumen Fermentation, and Microbial Community in anFrontiers in veterinary science · 2020Article
- Calcium salts of long-chain fatty acids from linseed oil decrease methane production by altering the rumen microbiome in vitro.PloS one · 2020Article
- Changes in the Rumen Microbiota of Cows in Response to Dietary Supplementation with Nitrate, Linseed, and Saponin Alone or in Combination.Applied and environmental microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dietary supplementation with linseed, saponins, and nitrate is a promising methane mitigation strategy in ruminant production. Here, we aimed to assess the effects of these additives on the rumen microbiota in order to understand underlying microbial mechanisms of methane abatement. Two 2-by-2 factorial design studies were conducted simultaneously, which also allowed us to make a broad-based assessment of microbial responses. Eight nonlactating cows were fed diets supplemented with linseed or saponin in order to decrease hydrogen production and nitrate to affect hydrogen consumption; also, combinations of linseed plus nitrate or saponin plus nitrate were used to explore the interaction between dietary treatments. Previous work assessed effects on methane and fermentation patterns. Rumen microbes were studied by sequencing 18S and 16S rRNA genes and ITS1 amplicons. Methanogen activity was monitored by following changes in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.