Evidence map›Paper›PMID 30502091›Full record

ArticleBiochemical and biophysical research communications2019

The role of extracellular matrix stiffness in regulating cytoskeletal remodeling via vinculin in synthetic smooth muscle cells.

Kai Shen, Harshavardhan Kenche, Hua Zhao, Jinping Li, Jasimine Stone

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Kai ShenDepartment of Chemistry and Forensic Science, Savannah State University, Savannah, GA, 31404, USA. Electronic address: shenk@savannahstate.edu.
Harshavardhan KencheDepartment of Chemistry and Forensic Science, Savannah State University, Savannah, GA, 31404, USA.
Hua ZhaoDepartment of Chemistry and Biochemistry, University of Northern Colorado, Greeley, CO, 80639, USA.
Jinping LiDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA, 31404, USA.
Jasimine StoneDepartment of Chemistry and Forensic Science, Savannah State University, Savannah, GA, 31404, USA.
Savannah State University · USMercer University · USUniversity of Northern Colorado · US

Funding

Metavinculin Regulation of Cell Cytoskeleton Remodeling in Response to SubstrateSC2GM112549 · NIGMS · SAVANNAH STATE UNIVERSITY · PI SHEN, KAI · 2015 to 2017
$297k
NIGMS NIH HHS SC2 GM112549
6 · The paper itself

Abstract

Vinculin is a key player in sensing and responding to external mechanical cues such as extracellular matrix stiffness. Increased matrix stiffness is often associated with certain pathological conditions including hypertension induced cellular cytoskeleton changes in vascular smooth muscle (VSM) cells. However, little is known on how stiffness affects cytoskeletal remodeling via vinculin in VSM cells. Thus, we utilized matrices with elastic moduli that simulate vascular stiffness in different stages of hypertension to investigate how matrix stiffness regulates cell cytoskeleton via vinculin in synthetic VSM cells. Through selecting a suitable reference gene, we found that an increase in physiologically relevant extracellular matrix stiffness (2-50 kPa) downregulates vinculin gene expression but upregulates vinculin protein expression. This discrepancy, which was not observed previously for non-muscle cells, suggests that the vinculin-mediated mecahnotransduction mechanism in synthetic VSM cells may be more complex than those proposed for non-muscle cells. Also adding to previous findings, we found that VSM cell growth may be impeded by substrates that are either too soft or too rigid.

Indexed as

AnimalsCells, CulturedCytoskeletonExtracellular MatrixMyocytes, Smooth MuscleRatsVinculinVcl protein, ratVinculinExtracellular matrixStiffnessSynthetic vascular smooth muscle cellsVinculin

Identifiers

PMID30502091
PMCPMC6382458
OpenAlexW2902838879

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.