Evidence map›Paper›PMID 30502051›Full record

ArticleEBioMedicine2019

Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency.

José C de-Lima-Júnior, Gabriela F Souza, Alexandre Moura-Assis, Rodrigo S Gaspar, Joana M Gaspar, Andréa L Rocha, Danilo L Ferrucci, Tanes I Lima, Sheila C Victório, Ivan L P Bonfante and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 41 citations in OpenAlex.

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  14. Regulation of mitochondrial temperature in health and disease.Pflugers Archiv : European journal of physiology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 2 institutions in 1 country.

José C de-Lima-JúniorLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Gabriela F SouzaLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Alexandre Moura-AssisLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Rodrigo S GasparObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; CEPECE - Research Center of Sport Sciences, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil(.).
Joana M GasparLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Andréa L RochaDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil.
Danilo L FerrucciDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil; National Institute of Photonics Applied to Cell Biology (INFABiC), Campinas, São Paulo, Brazil.
Tanes I LimaObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil.
Sheila C VictórioLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Ivan L P BonfanteLaboratory of Exercise Physiology, School of Physical Education, University of Campinas, Campinas, SP 13083-970, Brazil.
Claudia R CavaglieriLaboratory of Exercise Physiology, School of Physical Education, University of Campinas, Campinas, SP 13083-970, Brazil.
José C ParejaLaboratory of Investigation in Metabolism and Diabetes (LIMED)/Gastrocentro, Department of Surgery, University of Campinas (UNICAMP), Campinas, SP 13081-970, Brazil.
Sérgio Q BrunettoBiomedical Engineering Center, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Celso D RamosObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Radiology, University of Campinas, Campinas, São Paulo 13084-970, Brazil.
Bruno GelonezeObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Laboratory of Investigation in Metabolism and Diabetes (LIMED)/Gastrocentro, Department of Surgery, University of Campinas (UNICAMP), Campinas, SP 13081-970, Brazil.
Marcelo A MoriDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil.
Leonardo R SilveiraObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP 13083-970, Brazil.
Gesmar R S SegundoDepartment of Pediatrics, Federal University of Uberlandia, Uberlandia, Brazil.
Eduardo R RopelleObesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil; CEPECE - Research Center of Sport Sciences, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil(.).
Lício A VellosoLaboratory of Cell Signaling, Department of Internal Medicine, University of Campinas, Campinas, São Paulo 13084-970, Brazil; Obesity and Comorbidities Research Center, University of Campinas, Campinas, São Paulo 13084-970, Brazil. Electronic address: lavelloso@fcm.unicamp.br.
Universidade Estadual de Campinas (UNICAMP) · BRUniversidade Federal de Uberlândia · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInflammation is the most relevant mechanism linking obesity with insulin-resistance and metabolic disease. It impacts the structure and function of tissues and organs involved in metabolism, such as the liver, pancreatic islets and the hypothalamus. Brown adipose tissue has emerged as an important component of whole body energy homeostasis, controlling caloric expenditure through the regulation of non-shivering thermogenesis. However, little is known about the impact of systemic inflammation on the structure and function of brown adipose tissue.

methodsThe relations between IL10 and mitochondria structure/function and also with thermogenesis were evaluated by bioinformatics using human and rodent data. Real-time PCR, immunoblot, fluorescence and transmission electron microscopy were employed to determine the effect of IL10 in the brown adipose tissue of wild type and IL10 knockout mice.

findingsIL10 knockout mice, a model of systemic inflammation, present severe structural abnormalities of brown adipose tissue mitochondria, which are round-shaped with loss of cristae structure and increased fragmentation. IL10 deficiency leads to newborn cold intolerance and impaired UCP1-dependent brown adipose tissue mitochondrial respiration. The reduction of systemic inflammation with an anti-TNFα monoclonal antibody partially rescued the structural but not the functional abnormalities of brown adipose tissue mitochondria. Using bioinformatics analyses we show that in both humans and mice, IL10 transcripts correlate with mitochondrial lipid metabolism and caspase gene expression.

interpretationIL10 and systemic inflammation play a central role in the regulation of brown adipose tissue by controlling mitochondrial structure and function. FUND: Sao Paulo Research Foundation grant 2013/07607-8.

Indexed as

Adipose Tissue, BrownAnimalsCaspasesCell LineCold TemperatureComputational BiologyEnergy MetabolismGene Knockout TechniquesHumansInflammationInterleukin-10Lipid MetabolismMaleMiceMitochondriaShiveringCaspasesIL10 protein, humanInterleukin-10UCP1 protein, humanUncoupling Protein 1InflammationInterleukin-10MitochondriaObesityRespirationThermogenesis

Identifiers

PMID30502051
PMCPMC6355943
OpenAlexW2902911077

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.