Evidence map›Paper›PMID 30500333›Full record

ArticleDevelopmental biology2019

Developmental origins of transgenerational sperm DNA methylation epimutations following ancestral DDT exposure.

Millissia Ben Maamar, Eric Nilsson, Ingrid Sadler-Riggleman, Daniel Beck, John R McCarrey, Michael K Skinner

Abstract read
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In one paragraph

Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
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  7. Review
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  9. Article
  10. Redox mechanisms of environmental toxicants on male reproductive function.Frontiers in cell and developmental biology · 2024
    Review
  11. Review
  12. Review
  13. Article
  14. Transgenerational Inheritance and Systemic Racism in America.Psychiatric research and clinical practice · 2023
    Review
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  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Millissia Ben MaamarCenter for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA.
Eric NilssonCenter for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA.
Ingrid Sadler-RigglemanCenter for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA.
Daniel BeckCenter for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA.
John R McCarreyDepartment of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.
Michael K SkinnerCenter for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA. Electronic address: skinner@wsu.edu.

Funding

EPIGENETIC TRANSGENERATIONAL ENDOCRINE DISRUPTOR ACTIONSR01ES012974 · NIEHS · WASHINGTON STATE UNIVERSITY · PI SKINNER, MICHAEL K · 2005 to 2018
$4.6M
NIEHS NIH HHS R01 ES012974
6 · The paper itself

Abstract

Epigenetic alterations in the germline can be triggered by a number of different environmental factors from diet to toxicants. These environmentally induced germline changes can promote the epigenetic transgenerational inheritance of disease and phenotypic variation. In previous studies, the pesticide DDT was shown to promote the transgenerational inheritance of sperm differential DNA methylation regions (DMRs), also called epimutations, which can in part mediate this epigenetic inheritance. In the current study, the developmental origins of the transgenerational DMRs during gametogenesis have been investigated. Male control and DDT lineage F3 generation rats were used to isolate embryonic day 16 (E16) prospermatogonia, postnatal day 10 (P10) spermatogonia, adult pachytene spermatocytes, round spermatids, caput epididymal spermatozoa, and caudal sperm. The DMRs between the control versus DDT lineage samples were determined at each developmental stage. The top 100 statistically significant DMRs at each stage were compared and the developmental origins of the caudal epididymal sperm DMRs were assessed. The chromosomal locations and genomic features of the different stage DMRs were analyzed. Although previous studies have demonstrated alterations in the DMRs of primordial germ cells (PGCs), the majority of the DMRs identified in the caudal sperm originated during the spermatogonia stages in the testis. Interestingly, a cascade of epigenetic alterations initiated in the PGCs is required to alter the epigenetic programming during spermatogenesis to obtain the sperm epigenetics involved in the epigenetic transgenerational inheritance phenomenon.

Indexed as

AnimalsDDTDNA MethylationEpigenesis, GeneticFemaleInheritance PatternsMaleMutagensMutationPesticidesPregnancyRatsRats, Sprague-DawleySpermatogenesisSpermatozoaDDTMutagensPesticidesDNA methylationEpigeneticEpimutationPrimordial germ cellsSpermSpermatogenesisTestisTransgenerational

Identifiers

PMID30500333
PMCPMC6338333

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.