ArticlePlant cell reports2019
Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.).
Article in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- Transcriptomic analyses reveal regulatory plasticity and metabolic reprogramming underlying genotype-specific microspore embryogenesis in wheat.Plant cell reports · 2026Article
- Isolated Microspore Cultures of Triticale (× Triticosecale Wittm.).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Efficient Protocol for Doubled Haploid Production in Rye (Secale cereale L.).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transcriptomic identification of potential antioxidative enzyme regulators of the gametophytic-to-embryogenic switch in barley microspores.Frontiers in plant science · 2025Article
- β-glucans, SAM, and GSH fluctuations in barley anther tissue culture conditions affect regenerants' DNA methylation and GPRE.BMC plant biology · 2024Article
- New Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines.Plants (Basel, Switzerland) · 2024Article
- An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants.BMC plant biology · 2024Article
- Induction of Triticale (×Life (Basel, Switzerland) · 2023Article
- Article
- Triticale doubled haploid plant regeneration factors linked by structural equation modeling.Journal of applied genetics · 2022Article
- Article
- Physiological aspects of sex differences and Haldane's rule in Rumex hastatulus.Scientific reports · 2022Article
- Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticaleFrontiers in plant science · 2022Article
- Integrated Proteomic and Metabolomic Analyses Provide Insights Into Acquisition of Embryogenic Ability inFrontiers in plant science · 2022Article
- Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment.Scientific reports · 2021Article
- Impact of Ascorbate-Glutathione Cycle Components on the Effectiveness of Embryogenesis Induction in Isolated Microspore Cultures of Barley and Triticale.Antioxidants (Basel, Switzerland) · 2021Article
- Herbal Additives Substantially Modify Antioxidant Properties and Tocopherol Content of Cold-Pressed Oils.Antioxidants (Basel, Switzerland) · 2021Article
- Species with Haploid or Doubled Haploid Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageDepending on the capability for stress adaptation, the role played by glutathione in microspore embryogenesis consists of both antioxidative activity and stimulation of embryo-like structure development. The efficiency of microspore embryogenesis (ME) is determined by the complex network of internal and environmental factors. Among them, the efficient defence against oxidative stress seems to be one of the most important. The present study confirms this hypothesis showing the positive effect of glutathione-the most abundant cellular antioxidant-on ME in isolated microspore cultures of triticale (× Triticosecale Wittm.). For the first time, low temperature (LT) pre-treatment of tillers was combined with the exogenous application of glutathione and associated with the total activity of low-molecular weight antioxidants, the endogenous content and redox status of glutathione, and the effectiveness of ME. The results indicate that efficient antioxidative defence is the first, although not the only, prerequisite for effective ME. In responsive genotypes, LT alone stimulated antioxidative defence and decreased cell redox status, which was associated with increased cell viability and high frequency (ca. 20%) of microspore reprogramming. Application of glutathione had no effect either on the microspore viability or on the initial number of embryogenic microspores. However, it increased the number of embryo-like structures, probably by stimulating the next phases of its development. In recalcitrant genotypes, the main role of glutathione seems to be its participation in cell protection from oxidative stress. However, even enhanced antioxidative activity, which sustained cell viability and increased the number of embryogenic microspores, was insufficient for efficient haploid/doubled haploid plant production. Evidently, there are still other defective elements in the complex network of factors that regulate the process of ME.
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