ArticleAnalytical and bioanalytical chemistry2019
Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Development of a Rapid Isothermal Assay for Detection of Adenovirus Types Important in Respiratory Infections.Influenza and other respiratory viruses · 2025Article
- An experimental comparison between primer and nucleotide labelling to produce RPA-amplicons used for multiplex detection of antibiotic resistance genes.Scientific reports · 2023Article
- Toward Continuous Molecular Testing Using Gold-Coated Threads as Multi-Target Electrochemical Biosensors.Biosensors · 2023Article
- Trends of respiratory virus detection in point-of-care testing: A review.Analytica chimica acta · 2023Review
- Paper-Based Vertical Flow Immunoassay for the Point-of-Care Multiplex Detection of Radiation Dosimetry Genes.Cytogenetic and genome research · 2023Article
- Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics.The Analyst · 2022Article
- Design of Gold Nanoparticle Vertical Flow Assays for Point-of-Care Testing.Diagnostics (Basel, Switzerland) · 2022Review
- Advanced Signal-Amplification Strategies for Paper-Based Analytical Devices: A Comprehensive Review.Biomedicines · 2021Review
- Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests.Scientific reports · 2021Article
- Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Rapid Detection and Differentiation ofFrontiers in chemistry · 2021Article
- Microfluidic-based virus detection methods for respiratory diseases.Emergent materials · 2021Review
- Point-of-Care Approaches for Meningitis Diagnosis in a Low-Resource Setting (Southwestern Uganda): Observational Cohort Study Protocol of the "PI-POC" Trial.JMIR research protocols · 2020Article
- Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments.Mikrochimica acta · 2019Review
- Introducing a New Algorithm for Classification of Etiology in Studies on Pediatric Pneumonia: Protocol for the Trial of Respiratory Infections in Children for Enhanced Diagnostics Study.JMIR research protocols · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Respiratory viral infections often mimic the symptoms of infections caused by bacteria; however, restricted and targeted administration of antibiotics is needed to combat growing antimicrobial resistance. This is particularly relevant in low-income settings. In this work, we describe the use of isothermal amplification of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles. Two oligonucleotide probes, one in-house designed and one known adenoviral probe were tested and validated for microarray detection down to 50 nM using a synthetic target template. Furthermore, primers were shown to function in a recombinase polymerase amplification reaction using both synthetic template and viral DNA. As a proof-of-concept, we demonstrate adenoviral detection with four different adenoviral species associated with respiratory infections using the paper-based VFM format. The presented assay was validated with selected adenoviral species using the in-house probe, enabling detection at 1 ng of starting material with intra- and inter-assay %CV of ≤ 9% and ≤ 13%. Finally, we validate our overall method using clinical samples. Based on the results, the combination of recombinase polymerase amplification, paper microarray analysis, and nanoparticle-based colorimetric detection could thus be a useful strategy towards rapid and affordable multiplexed viral diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.