Evidence map›Paper›PMID 30498222›Full record

ArticleNature communications2018

Engineering Saccharomyces cerevisiae for co-utilization of D-galacturonic acid and D-glucose from citrus peel waste.

Ryan J Protzko, Luke N Latimer, Ze Martinho, Elise de Reus, Tanja Seibert, J Philipp Benz, John E Dueber

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 98 citations in OpenAlex.

  1. Review
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  16. Review
  17. Use of ambrAMB Express · 2022
    Article
  18. Microbial valorization of underutilized and nonconventional waste streams.Journal of industrial microbiology & biotechnology · 2022
    Review
  19. Process biochemistry (Barking, London, England) · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Ryan J ProtzkoDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.
Luke N LatimerDepartment of Chemistry, University of California, Berkeley, CA, 94720, USA.
Ze MartinhoDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA.
Elise de ReusDepartment of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, Denmark.
Tanja SeibertHolzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising, Germany.
J Philipp BenzHolzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising, Germany.ORCID http://orcid.org/0000-0001-5361-4514
John E DueberDepartment of Bioengineering, University of California, Berkeley, CA, 94720, USA. jdueber@berkeley.edu.ORCID http://orcid.org/0000-0001-8404-7153
University of California, Berkeley · USLawrence Berkeley National Laboratory · USMax Planck Institute of Molecular Plant Physiology · DETechnical University of Denmark · DKTechnical University of Munich · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished inexpensively to yield high concentrations of fermentable sugars and D-galacturonic acid (D-galUA). In this study, we tackle a number of challenges associated with engineering a microbial strain to convert pectin-rich hydrolysates into commodity and specialty chemicals. First, we engineer D-galUA utilization into yeast, Saccharomyces cerevisiae. Second, we identify that the mechanism of D-galUA uptake into yeast is mediated by hexose transporters and that consumption of D-galUA is inhibited by D-glucose. Third, we enable co-utilization of D-galUA and D-glucose by identifying and expressing a heterologous transporter, GatA, from Aspergillus niger. Last, we demonstrate the use of this transporter for production of the platform chemical, meso-galactaric acid, directly from industrial Navel orange peel waste.

Indexed as

Aspergillus nigerCitrusFermentationGlucoseHexuronic AcidsSaccharomyces cerevisiaegalacturonic acidGlucoseHexuronic Acids

Identifiers

PMID30498222
PMCPMC6265301
OpenAlexW2901131716

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.