ArticleBMC plant biology2018
In vivo haploid induction leads to increased frequency of twin-embryo and abnormal fertilization in maize.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- An all-in-one visual selection system for male-sterile line production in maize and rice.Plant communications · 2026Article
- Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes.International journal of molecular sciences · 2025Review
- Maize In Planta Haploid Inducer Lines: A Cornerstone for Doubled Haploid Technology.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Puzzling out plant reproduction by haploid induction for innovations in plant breeding.Nature plants · 2020Review
- Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.).PloS one · 2020Article
- Doubled haploid technology for line development in maize: technical advances and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
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8 authors.
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Abstract
backgroundIn vivo haploid induction (HI) based on Stock6-derived inducer lines has been the most prevalent means of producing haploids. Nevertheless, the biological mechanism of HI is not fully understood, the twin-embryo kernels had been found during haploid induction, which may provide potential evidence for the abnormal double fertilization during HI.
resultsWe investigated twin-embryo frequency in progenies of different haploid inducers. Results reveal that increasing the HI potential significantly improved the frequency of twin-embryo kernels. Compared with the average twin-embryo kernel frequency (average frequency = 0.07%) among progenies pollinated by the haploid inducer line CAUHOI, the frequency of twin-embryo was improved to 0.16% in progenies pollinated by the haploid inducer line CAU5. This result was further confirmed by pollinating single hybrid ND5598 with four haploid inducers possessing differentiated HIRs, where twin-embryo frequency was highly correlated with HIR. Among 237 twin-embryo kernels, we identified 30 haploid twin-embryo kernels (12.66%), a frequency which was much greater than the average HI rate for three other inducer lines (frequency range 2-10%). In addition, aneuploids, occurred at high frequency (8 in 41 twin plants). This level of aneuploidy provides new insight into the abnormal double fertilization during HI. Moreover, we observed differences in growth rate between twin plants in the field, as 4.22% of the twin plants grew at a significantly different rate. Both simple sequence repeats markers (SSR) and 3072 SNP-chip genotyping results revealed that > 90% of the twin plants shared the same origin, and the growth difference could be attributed to aneuploidy, competition for nutrients, and possible hormone regulation.
conclusionThese results demonstrate that an enhanced HI ability can increase twin-embryo kernel frequency, and high frequency of both haploid twin-embryo kernels and aneuploidy observed in this research give us new insights to understand the mechanism of both HI and abnormal embryogenesis.
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