ArticleJournal of proteome research2019
Discovery and Quantification of Nonhuman Proteins in Human Milk.
Article in Journal of proteome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- From the Field - Supporting Families as They Navigate Infant Food Allergies.Journal of human lactation : official journal of International Lactation Consultant Association · 2026Review
- Enrichment of Low-Abundance and Low-Molecular-Weight Proteins in Human Milk Using Mass Spectrometry-Based Proteomics.ACS omega · 2025Article
- A long-lasting prolactin stimulates galactopoiesis in mice.iScience · 2025Article
- The ferroform and glycoform landscape of human milk lactoferrin dissected through hybrid mass spectrometric approaches.Frontiers in nutrition · 2025Article
- Detection of bovine milk-, but likely not soy-derived, peptides in human milk after maternal consumption of bovine milk and soy beverage: a randomized, cross-over, dietary intervention trial.Frontiers in nutrition · 2025Article
- Review
- Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides releasedFrontiers in pharmacology · 2024Article
- Effect of UHT Thermal Treatment on the Secondary Structures of Milk Proteins: Insights From FTIR Analysis and Potential Allergenic Activity.International journal of food science · 2024Article
- Shaping infant development from the inside out: Bioactive factors in human milk.Seminars in perinatology · 2023Review
- Precision diagnostics in children.Cambridge prisms. Precision medicine · 2023Review
- Comparative proteomics of human milk casein fraction collected from women of Korean and Han ethnic groups in China.Frontiers in nutrition · 2023Article
- Allergen Content and Protease Activity in Milk Feeds from Mothers of Preterm Infants.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2022Observational
- Cow's Milk Antigens Content in Human Milk: A Scoping Review.Foods (Basel, Switzerland) · 2022Article
- Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Exploring Human Milk Dynamics: Interindividual Variation in Milk Proteome, Peptidome, and Metabolome.Journal of proteome research · 2022Article
- Soluble HLA peptidome: A new resource for cancer biomarkers.Frontiers in oncology · 2022Review
- The human milk proteome and allergy of mother and child: Exploring associations with protein abundances and protein network connectivity.Frontiers in immunology · 2022Article
- Quantification of FAM20A in human milk and identification of calcium metabolism proteins.Physiological reports · 2021Article
- Monitoring Human Milk β-Casein Phosphorylation andInternational journal of molecular sciences · 2021Article
- Provider recommendations and maternal practices when providing breast milk to children with immunoglobulin E-mediated food allergy.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The question whether and which nonhuman peptides or proteins are present in human milk was raised many decades ago. However, due to cross-reactivity or nonspecific antibody recognition, the accuracy of detection by immunochemical methods has been a concern. Additionally, the relative low-abundance of nonhuman peptides/proteins in the complex milk sample makes them a challenging target to detect. Here, by deep proteome profiling, we detected several nonhuman peptides, which could be grouped as nonhuman proteins. We next estimated their concentration in human milk by combining data-dependent shotgun proteomics and parallel reaction monitoring. First, we fractionated human milk at the protein level and were able to detect 1577 human proteins. Additionally, we identified 109 nonhuman peptides, of which 71 were grouped into 36 nonhuman proteins. In the next step, we targeted 37 nonhuman peptides and nine of them could be repeatedly quantified in human milk samples. Peptides/proteins originating from bovine milk products were the dominant nonhuman proteins observed, notably bovine caseins (α-S1-, α-S2-, β-, κ-caseins) and β-lactoglobulin. The method we present here can be expanded to investigate more about nonhuman peptides and proteins in human milk and give a better understanding of how human milk plays a role in allergy prevention.
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