ArticleThe Journal of experimental medicine1978
Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro.
Article in The Journal of experimental medicine, 1978. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 316 citations in OpenAlex.
- Steroid resistance in Diamond Blackfan anemia associates with p57Kip2 dysregulation in erythroid progenitors.The Journal of clinical investigation · 2020Article
- Genetically Engineered In Vitro Erythropoiesis.International journal of stem cells · 2016Article
- Exogenous c-Myc Blocks Differentiation and Improves Expansion of Human Erythroblasts In vitro.International journal of stem cells · 2014Article
- Article
- Effect of cimetidine on burst-promoting activity of normal T lymphocytes.Annals of hematology · 1992Article
- The Jeremiah Metzger lecture. Regulation of hematopoiesis.Transactions of the American Clinical and Climatological Association · 1990Review
- Human colony-forming units-erythroid do not require accessory cells, but do require direct interaction with insulin-like growth factor I and/or insulin for erythroid development.The Journal of clinical investigation · 1989Article
- Nerve growth factor promotes human hemopoietic colony growth and differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Hematopoietic growth factors.The Journal of clinical investigation · 1987Article
- Interleukin 1 induces cultured human endothelial cell production of granulocyte-macrophage colony-stimulating factor.The Journal of clinical investigation · 1987Article
- Gamma interferon and lymphotoxin, released by activated T cells, synergize to inhibit granulocyte/monocyte colony formation.The Journal of experimental medicine · 1986Article
- Dependence of highly enriched human bone marrow progenitors on hemopoietic growth factors and their response to recombinant erythropoietin.The Journal of clinical investigation · 1986Article
- A cellular analysis of long-term haematopoietic damage in mice after repeated treatment with cyclophosphamide.Cancer chemotherapy and pharmacology · 1986Article
- Suppression of normal human erythropoiesis by gamma interferon in vitro. Role of monocytes and T lymphocytes.The Journal of clinical investigation · 1985Article
- Identification of three accessory cell populations in human bone marrow with erythroid burst-promoting properties.The Journal of clinical investigation · 1985Article
- Article
- A monokine stimulates production of human erythroid burst-promoting activity by endothelial cells in vitro.The Journal of clinical investigation · 1985Article
- Interferon is a mediator of hematopoietic suppression in aplastic anemia in vitro and possibly in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1985Article
- Pathology-important advances in clinical medicine: assessing fetal lung maturity.The Western journal of medicine · 1984Article
- Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.Proceedings of the National Academy of Sciences of the United States of America · 1984Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human mononuclear leukocytes were fractionated into populations of null, T and B cells by immunoabsorbent column chromatography followed by E-rosette formation and purification of T cells by differential centrifugation and osmotic lysis. The unfractionated and fractionated cell populations were first separately cultured for 14 days in plasma clots in the presence of two international units erythropoietin. Typical erythroid burst-forming unit (BFU-E)-derived colonies grew in the unfractionated cell cultures but not from T- or B-cell cultures. BFU-E colonies grew in null cell cultures but most of the colonies were small and variably hemoglobinized with less than three subcolonies. When intact T cells were added to null cells and cocultured, many typical large BFU-E colonies with more than 10 well homogenized subcolonies appeared. Increasing numbers of large BFU-E colonies in null cell cultures were induced by stepwise addition of T cells but not by the addition of B cells. A conditioned medium in which T cells had been induced to divide by tetanus toxoid substituted for intact T cells in this T-cell-dependent BFU-E colony formation observed in null cells. These findings demonstrate that the BFU-E, a committeded erythroid stem cell, resides in the null cell fraction of peripheral blood, but its proliferative capacity and differentiation in vitro requires a soluble product of T cells. Such experiments now permit a new approach to the assessment of various disorders of erythropoiesis. Erythroid hypoplasia in a particular case may be due to dysfunction of the committed precursor cell or to a failure of a helper effect induced by T cells.
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