ArticleMicrobial cell factories2018
Highly efficient production of Clostridium cellulolyticum H10 D-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce D-psicose.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 39 citations in OpenAlex.
- D-Allulose Reduces Weight More Persistently than Oral Semaglutide While Both Equally Elevate Grip Strength in Diet-Induced Obese Mice.Nutrients · 2026Article
- Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.Scientific reports · 2024Article
- The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability.International journal of molecular sciences · 2024Article
- The Engineering, Expression, and Immobilization of Epimerases forInternational journal of molecular sciences · 2023Review
- Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose.Frontiers in microbiology · 2022Review
- Engineering ofFrontiers in bioengineering and biotechnology · 2022Article
- Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021Review
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis.Applied and environmental microbiology · 2020Article
- Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundD-Psicose 3-epimerase (DPEase) catalyzes the isomerization of D-fructose to the rare sugar D-psicose, which may help prevent obesity, reduce blood sugar and blood fat, and inhibit intra-abdominal fat accumulation.
resultsIn this study, the DPEase of Clostridium cellulolyticum H10 was expressed in the food-grade host Bacillus subtilis. Optimization of the culture medium during shake-flask experiments yielded a DPEase activity of 314 U/mL. The optimal medium included 20 g/L peptone, 15 g/L corn steep powder, 5 g/L glycerol, and 1 mM Ca
conclusionsThis is the first report to enhance recombinant DPEase production in B. subtilis using efficient and convenient fermentation strategy, and the DPEase yield is three times higher than the highest yield reported to date. The recombinant B. subtilis cells were further used in the efficient synthesis of D-psicose. This study provides a basis for the industrial production of D-psicose.
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