ArticleMicrobial cell factories2018
The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Genome Mining-Guided Discovery of the Glycosylated Griseorhodin Congener Ruskamycin.Journal of natural products · 2026Article
- A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites.ACS synthetic biology · 2026Article
- Rheology and Culture Reproducibility of Filamentous Microorganisms: Impact of Flow Behavior and Oxygen Transfer During Salt-Enhanced Cultivation of the ActinomyceteEngineering in life sciences · 2025Article
- Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations.Engineering in life sciences · 2025Review
- Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad inSynthetic and systems biotechnology · 2024Article
- Prodigiosin: unveiling the crimson wonder - a comprehensive journey from diverse bioactivity to synthesis and yield enhancement.Frontiers in microbiology · 2024Review
- Characterization of Bioactive Colored Materials Produced from Bacterial Cellulose and Bacterial Pigments.Materials (Basel, Switzerland) · 2022Article
- Antimicrobial Food Packaging Based on Prodigiosin-Incorporated Double-Layered Bacterial Cellulose and Chitosan Composites.Polymers · 2022Article
- Customized chitooligosaccharide production-controlling their lengthFrontiers in bioengineering and biotechnology · 2022Article
- A Genome-Scale Insight into the Effect of Shear Stress During the Fed-Batch Production of Clavulanic Acid byMicroorganisms · 2020Article
- Impact of Oxygen Supply and Scale Up onFrontiers in bioengineering and biotechnology · 2020Article
- Characterization of the Metabolic Response ofAntibiotics (Basel, Switzerland) · 2019Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundIn Streptomyces, understanding the switch from primary to secondary metabolism is important for maximizing the production of secondary metabolites such as antibiotics, as well as for optimizing recombinant glycoprotein production. Differences in Streptomyces lividans bacterial aggregation as well as recombinant glycoprotein production and O-mannosylation have been reported due to modifications in the shake flask design. We hypothetized that such differences are related to the metabolic switch that occurs under oxygen-limiting conditions in the cultures.
resultsShake flask design was found to affect undecylprodigiosin (RED, a marker of secondary metabolism) production; the RED yield was 12 and 385 times greater in conventional normal Erlenmeyer flasks (NF) than in baffled flasks (BF) and coiled flasks (CF), respectively. In addition, oxygen transfer rates (OTR) and carbon dioxide transfer rates were almost 15 times greater in cultures in CF and BF as compared with those in NF. Based on these data, we obtained respiration quotients (RQ) consistent with aerobic metabolism for CF and BF, but an RQ suggestive of anaerobic metabolism for NF.
conclusionAlthough the metabolic switch is usually related to limitations in phosphate and nitrogen in Streptomyces sp., our results reveal that it can also be activated by low OTR, dramatically affecting recombinant glycoprotein production and O-mannosylation and increasing RED synthesis in the process.
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