Evidence map›Paper›PMID 30486430›Full record

ArticleInternational journal of molecular sciences2018

Efficient and Stable Delivery of Multiple Genes to Fish Cells by a Modified Recombinant Baculovirus System.

Qian Wang, Jian Fang, Qihua Pan, Yizhou Wang, Ting Xue, Lingyu Li, Tiansheng Chen

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Qian WangKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. qian.wang@webmail.hzau.edu.cn.
Jian FangKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. 15527598323@163.com.
Qihua PanKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. panqihua@webmail.hzau.edu.cn.
Yizhou WangKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. 18627944916@163.com.
Ting XueKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. xue2011ting124@126.com.
Lingyu LiKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. lilingyu@webmail.hzau.edu.cn.
Tiansheng ChenKey Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. chen.tiansheng@mail.hzau.edu.cn.ORCID 0000-0003-4763-2307
Huazhong Agricultural University · CN

Funding

Huazhong Agricultural University Scientific & Technological Self-innovation Foundation 2013RC014National Key Basic Research Program of China 2013CB967700National Natural Science Foundation of China 31672653National Natural Science Foundation of China 31771648
6 · The paper itself

Abstract

The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-early gene 1 (ie1) (WSSV ie1), followed by a puromycin⁻green fluorescent protein (Puro-GFP,

Indexed as

Gene ExpressionTransgenesAnimalsBaculoviridaeCell LineFishesGene OrderGenes, ReporterGenetic VectorsGene Transfer TechniquesGreen Fluorescent ProteinsPromoter Regions, GeneticRecombinant ProteinsGreen Fluorescent ProteinsRecombinant Proteinsfast titer determinationfish cellsrecombinant baculovirusstable gene deliveryWSSV ie1 promoter

Identifiers

PMID30486430
PMCPMC6321422
OpenAlexW2902974987

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.