Evidence map›Paper›PMID 30484293›Full record

ArticleJournal of biomedical optics2018

Penetration model for chemical reactivation for resin-embedded green fluorescent protein imaging.

Longhui Li, Ruixi Chen, Xiuli Liu, Ning Li, Xiaoxiang Liu, Xiaojun Wang, Tingwei Quan, Xiaohua Lv, Shaoqun Zeng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Longhui LiHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Ruixi ChenHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Xiuli LiuHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Ning LiHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Xiaoxiang LiuHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Xiaojun WangHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Tingwei QuanHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Xiaohua LvHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Shaoqun ZengHuazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan, China.
Wuhan National Laboratory for Optoelectronics · CNHuazhong University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the so-called surface microscopy, serial block-face imaging is combined with mechanic sectioning to obtain volumetric imaging. While mapping a resin-embedded green fluorescent protein (GFP)-labeled specimen, it has been recently reported that an alkaline buffer is used to chemically reactivate the protonated GFP molecules, and thus improve the signal-to-noise ratio. In such a procedure, the image quality is highly affected by the penetration rate of a solution. We propose a reliable penetration model to describe the penetration process of the solution into the resin. The experimental results are consistent with the parameters predicted using this model. Thus, this model provides a valuable theoretical explanation and aids in optimizing the system parameters for mapping resin-embedded GFP biological samples.

Indexed as

Green Fluorescent ProteinsHistological TechniquesImaging, Three-DimensionalLuminescent ProteinsMicroscopy, FluorescenceTissue EmbeddingGreen Fluorescent ProteinsLuminescent Proteinschemical reactivationfluorescence imagingpenetration modelresin embedding

Identifiers

PMID30484293
PMCPMC6992894
OpenAlexW2903455096

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.