ArticleMolecular biotechnology2019
Strategies for the Construction of Cassava Brown Streak Disease Viral Infectious Clones.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Viral inosine triphosphatase: A mysterious enzyme with typical activity, but an atypical function.Molecular plant pathology · 2021Review
- Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV.Plants (Basel, Switzerland) · 2020Article
- Review
- Utilization of infectious clones to visualize Cassava brown streak virus replication in planta and gain insights into symptom development.Virus genes · 2019Article
- Cassava brown streak virus Ham1 protein hydrolyses mutagenic nucleotides and is a necrosis determinant.Molecular plant pathology · 2019Article
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Authors and funding
11 authors.
Funding
Abstract
Cassava brown streak disease (CBSD) has major impacts on yield and quality of the tuberous roots of cassava in Eastern and Central Arica. At least two Potyviridae species cause the disease: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Cloned viral genome sequences known as infectious clones (ICs) have been important in the study of other viruses, both as a means of standardising infectious material and characterising viral gene function. IC construction is often technically challenging for Potyviridae due to sequence instability in E. coli. Here, we evaluate three methods for the construction of infectious clones for CBSD. Whilst a simple IC for in vitro transcription was made for UCBSV isolate 'Kikombe', such an approach failed to deliver full-length clones for CBSV isolates 'Nampula' or 'Tanza', necessitating more complex approaches for their construction. The ICs successfully generated symptomatic infection in the model host N. benthamiana and in the natural host cassava. This shows that whilst generating ICs for CBSV is still a technical challenge, a structured approach, evaluating both in vitro and in planta transcription systems should successfully deliver ICs, allowing further study into the symptomology and virulence factors in this important disease complex.
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Registered trials
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