ArticleBMC pediatrics2018
Necrotizing enterocolitis leads to disruption of tight junctions and increase in gut permeability in a mouse model.
Article in BMC pediatrics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Vitamins as modulators of neonatal intestinal injury.Frontiers in nutrition · 2026Review
- Tight junction proteins and intestinal health in preterm infants.Journal of neonatal-perinatal medicine · 2025Review
- Bedside Utilization of Intestinal Pathology in Preterm Infants with Surgical Necrotizing Enterocolitis.American journal of perinatology · 2025Review
- Review
- Human breast milk-derived exosomes and their positive role on neonatal intestinal health.Pediatric research · 2025Review
- Thrombin-preconditioned mesenchymal stromal cell-derived extracellular vesicles attenuate experimental necrotizing enterocolitis.Stem cell research & therapy · 2025Article
- Development of a refined neonatal rabbit model of necrotizing enterocolitis with relatively longer survival.Frontiers in physiology · 2025Article
- Therapeutic potential of human breast milk-derived exosomes in necrotizing enterocolitis.Molecular medicine (Cambridge, Mass.) · 2024Review
- Cannabidiol decreases histological intestinal injury in a neonatal experimental model of necrotizing enterocolitis.Laboratory animal research · 2024Article
- Outcomes by disease onset, sex, and intervention in neonates with SIP and surgical NEC.Pediatric research · 2024Article
- Clinical characteristics of postoperative necrotizing enterocolitis in patients with congenital jejunoileal atresia and its risk factors.World journal of pediatric surgery · 2024Article
- Interfering with Rac1-activation during neonatal monocyte-macrophage differentiation influences the inflammatory responses of M1 macrophages.Cell death & disease · 2023Article
- Article
- Microbial Interventions to Improve Neonatal Gut Health.Microorganisms · 2023Review
- State-of-the-art review and update ofFrontiers in pediatrics · 2023Review
- In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis.Journal of visualized experiments : JoVE · 2022Article
- Clinical outcomes and gestational age based prediction of pneumatosis intestinalis in preterm infants with necrotizing enterocolitis.Journal of neonatal-perinatal medicine · 2022Article
- Clinical implications of preterm infant gut microbiome development.Nature microbiology · 2022Article
- Saireito, a Japanese herbal medicine, alleviates leaky gut associated with antibiotic-induced dysbiosis in mice.PloS one · 2022Article
- Review of claudin proteins as potential biomarkers for necrotizing enterocolitis.Irish journal of medical science · 2021Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundNecrotizing enterocolitis (NEC) is a leading cause of death in preterm infants. Neonates weighing <1500 grams are at the highest risk for acquiring NEC, with a prevalence of nearly 7-10%, mortality up to 30%, and several long-term complications among survivors. Despite advancements in neonatal medicine, this disease remains a challenge to treat. The aim of this study is to investigate the effect of NEC on gut epithelial tight junctions and its barrier function using a NEC mouse model.
methodsThree-day old C57BL/6 mouse pups were fed with Esbilac formula every 3 hours and then subjected to hypoxia twice daily followed by cold stress. Dam fed pups from the same litters served as controls. Pups were observed and sacrificed 96 hours after the treatments and intestines were removed for experiments. The successful induction of NEC was confirmed by histopathology. Changes in tight junction proteins in NEC intestines were studied by western blotting and immunofluorescent microscopy using specific protein markers. The gut leakage in NEC was visualized using biotin tracer molecules.
resultsOur study results demonstrate that we induced NEC in >50% of experimental pups, pups lost nearly 40% of weight and their intestines showed gross changes and microscopic changes associated with NEC. There were inflammatory changes with loss of tight junction barrier function and disruption of tight junction claudin proteins in the intestines of NEC mouse model. We have demonstrated for the first time that NEC intestines develop increased leakiness as visualized by biotin tracer leakage.
conclusionsNEC leads to breakdown of epithelial barrier due to changes in tight junction proteins with increased leakiness which may explain the transmigration of microbes and microbial products from the gut lumen into the blood stream leading to sepsis like signs clinically witnessed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.