ArticleJournal of proteome research2019
TKO6: A Peptide Standard To Assess Interference for Unit-Resolved Isobaric Labeling Platforms.
Article in Journal of proteome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Adapting an Isobaric Tag-Labeled Yeast Peptide Standard to Develop Targeted Proteomics Assays.Journal of proteome research · 2024Article
- Isobaric labeling: Expanding the breadth, accuracy, depth, and diversity of sample multiplexing.Proteomics · 2022Article
- Assessing interference in isobaric tag-based sample multiplexing using an 18-plex interference standard.Proteomics · 2022Article
- Quantitative Accuracy and Precision in Multiplexed Single-Cell Proteomics.Analytical chemistry · 2022Review
- Proximity labeling for investigating protein-protein interactions.Methods in cell biology · 2022Article
- Strategies for mass spectrometry-based phosphoproteomics using isobaric tagging.Expert review of proteomics · 2021Review
- TMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing.Journal of proteome research · 2021Article
- Advances in quantitative high-throughput phosphoproteomics with sample multiplexing.Proteomics · 2021Review
- Strain-Specific Peptide (SSP) Interference Reference Sample: A Genetically Encoded Quality Control for Isobaric Tagging Strategies.Analytical chemistry · 2021Article
- HYpro16: A Two-Proteome Mixture to Assess Interference in Isobaric Tag-Based Sample Multiplexing Experiments.Journal of the American Society for Mass Spectrometry · 2021Article
- A Triple Knockout Isobaric-Labeling Quality Control Platform with an Integrated Online Database Search.Journal of the American Society for Mass Spectrometry · 2020Article
- Mass Spectrometry Advances and Perspectives for the Characterization of Emerging Adoptive Cell Therapies.Molecules (Basel, Switzerland) · 2020Review
- Optimized Workflow for Multiplexed Phosphorylation Analysis of TMT-Labeled Peptides Using High-Field Asymmetric Waveform Ion Mobility Spectrometry.Journal of proteome research · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Protein abundance profiling using isobaric labeling is a well-established quantitative mass spectrometry technique. However, ratio distortion resulting from coisolated and cofragmented ions, commonly referred to as interference, remains a drawback of this strategy. Tribrid mass spectrometers, such as the Orbitrap Fusion and the Orbitrap Fusion Lumos with a triple mass analyzer configuration, facilitate methods (namely, SPS-MS3) that can help alleviate interference. However, few standards are available to measure interference and thereby aid in method development. Here we introduce the TKO6 standard that assesses ion interference and is designed specifically for data acquired at low (unit) mass resolution. We use TKO6 to compare interference in MS2- versus MS3-based quantitation methods, data acquisition methods of different lengths, and ion-trap-based tandem mass tag reporter ion analysis (IT-MS3) with conventional Orbitrap-based analysis (OT-MS3). We show that the TKO6 standard is a valuable tool for assessing quantification accuracy in isobaric-tag-based analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.