ArticleTranslational vision science & technology2018
Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma.
Article in Translational vision science & technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Overview of cicatricial modulators in glaucoma fistulizing surgery.International ophthalmology · 2020Pooled it
- Effect of ripasudil after trabeculectomy with mitomycin C: a multicentre, randomised, prospective clinical study.BMJ open ophthalmology · 2024Trial
- The Strain Response to Intraocular Pressure Decrease in the Lamina Cribrosa of Patients with Glaucoma.Ophthalmology. GlaucomaTrial
- A Comparison of the Efficacy and Safety of Modified Baerveldt Drainage Implants versus Ahmed Glaucoma Valves After 1 Year of Follow-Up.Clinical ophthalmology (Auckland, N.Z.) · 2026Article
- Analysis of 2-dimensional regional differences in the peripapillary scleral fibroblast cytoskeleton of normotensive and hypertensive mouse eyes.Scientific reports · 2025Article
- New nanofiber glaucoma drainage implant: Effectiveness, safety, first in vivo results, and optimization of surgical technique.PloS one · 2025Article
- A Scoping Review of Rho Kinase Inhibitors and their Role in Glaucoma Treatment.Current medicinal chemistry · 2025Article
- Control of spatio-temporal patterning via cell growth in a multicellular synthetic gene circuit.Nature communications · 2024Article
- The Mechanisms of Neuroprotection by Topical Rho Kinase Inhibition in Experimental Mouse Glaucoma and Optic Neuropathy.Investigative ophthalmology & visual science · 2024Article
- IOP and glaucoma damage: The essential role of optic nerve head and retinal mechanosensors.Progress in retinal and eye research · 2024Review
- Glaucoma: Novel antifibrotic therapeutics for the trabecular meshwork.European journal of pharmacology · 2023Review
- Collagen Fibril Orientation Instructs Fibroblast Differentiation Via Cell Contractility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Nanofiber-based glaucoma drainage implant improves surgical outcomes by modulating fibroblast behavior.Bioengineering & translational medicine · 2023Article
- Review
- Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma:Drug delivery · 2022Article
- Quantitative Microstructural Analysis of Cellular and Tissue Remodeling in Human Glaucoma Optic Nerve Head.Investigative ophthalmology & visual science · 2022Article
- Ultrastructural analysis of explanted CyPass microstents and correlation with clinical findings.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2022Article
- Remodeling of the Lamina Cribrosa: Mechanisms and Potential Therapeutic Approaches for Glaucoma.International journal of molecular sciences · 2022Review
- Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.Journal of molecular and cellular cardiology · 2021Article
- Engineering biomaterials to prevent post-operative infection and fibrosis.Drug delivery and translational research · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeWe evaluated prevention of transforming growth factor β (TGFβ)-induced transdifferentiation of cultured scleral fibroblasts to myofibroblasts by rho-associated protein kinase (ROCK) inhibitors. Additionally, we tested whether local delivery of ROCK inhibitors reduced scleral fibroblast proliferation in response to chronic intraocular pressure (IOP) elevation.
methodsPrimary human peripapillary sclera (PPS) fibroblasts were cultured and treated with TGFβ to induce myofibroblast transdifferentiation, as determined by immunoblot assessment of α smooth muscle actin (SMA) levels and collagen gel contraction. Cells were treated with the ROCK inhibitors Y27632, fasudil, and H1152 before TGFβ treatment. ROCK activity in TGFβ-treated fibroblasts and sclera from ocular hypertensive mice was assessed by measuring phosphorylation of the ROCK substrate MYPT1 at Thr696. Fibroblast proliferation following IOP elevation and ROCK inhibitor treatment was assessed by an enzyme-linked immunosorbent (ELISA) assay.
resultsROCK inhibitors H1152 (10μM), Y27632 (10 μM), and fasudil (5μM) reduced SMA expression 72%, 85%, and 68%, respectively. Collagen gel contraction was reduced by 36% (
conclusionsROCK inhibitors reduce TGFβ-induced myofibroblast transdifferentiation and glaucoma-induced scleral cell proliferation. TRANSLATIONAL RELEVANCE: These findings suggest altered fibroblast activity promoted by ROCK inhibitors could modify scleral biomechanics and be relevant to glaucoma treatment.
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