Evidence map›Paper›PMID 30479711›Full record

ArticleJournal of ophthalmic & vision research

Protective Effects of 17β-Estradiol on Benzo(e) pyrene[B(e)P]-induced Toxicity in ARPE-19 cells.

Ashish U Sapkal, Sonali Nashine, Saffar Mansoor, Vishal R Sharma, Claudio A Ramirez, Rafael Z Migon, Navin K Gupta, Marilyn Chwa, Baruch D Kuppermann, M Cristina Kenney

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ophthalmic & vision research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Ashish U SapkalDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Sonali NashineDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Saffar MansoorDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Vishal R SharmaDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Claudio A RamirezDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Rafael Z MigonDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Navin K GuptaDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Marilyn ChwaDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
Baruch D KuppermannDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
M Cristina KenneyDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California, Irvine, CA, USA.
University of California, Irvine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells.

methodsWe pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured.

resultsOur results demonstrated that when treated with B(e)P, the viability and ΔΨm of ARPE-19 cells declined by 25% and 63%, respectively (

conclusionIn conclusion, our results demonstrate that 17β-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential.

Indexed as

17β-EstradiolAge-Related Macular DegenerationApoptosisBenzo(e)pyreneRetinal Pigment Epithelial CellsSmoking

Identifiers

PMID30479711
PMCPMC6210882
OpenAlexW2898290244

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.