ArticleBiotechnology for biofuels2018
Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 90 citations in OpenAlex.
- The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond.BMC plant biology · 2026Review
- A Plasma Membrane-Located Lysophosphatidic Acid Acyltransferase in Microalga Myrmecia incisa Prefers Arachidonic Acid-CoA to Produce Glycerolipids.Applied biochemistry and biotechnology · 2026Article
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Diatom triacylglycerol metabolism: from carbon fixation to lipid droplet degradation.Biological reviews of the Cambridge Philosophical Society · 2025Review
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Heat shock transcription factor-mediated thermal tolerance and cell size plasticity in marine diatoms.Nature communications · 2025Article
- Improving Lipid Content in the DiatomCurrent issues in molecular biology · 2024Article
- Purple acid phosphatase promoted hydrolysis of organophosphate pesticides in microalgae.Environmental science and ecotechnology · 2024Article
- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion.Microorganisms · 2023Review
- Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis.Plant physiology · 2023Article
- Bioinformatics-Based Screening Approach for the Identification and Characterization of Lipolytic Enzymes from the Marine DiatomMarine drugs · 2023Article
- Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica.Microbial cell factories · 2023Article
- Increased Lipids inInternational journal of molecular sciences · 2022Article
- Lysophosphatidic acid acyltransferase 2 and 5 commonly, but differently, promote seed oil accumulation in Brassica napus.Biotechnology for biofuels and bioproducts · 2022Article
- Enhancing microalgal lipid accumulation for biofuel production.Frontiers in microbiology · 2022Review
- Advanced Applications for Protein and Compounds from Microalgae.Plants (Basel, Switzerland) · 2021Review
- Bacterial diketopiperazines stimulate diatom growth and lipid accumulation.Plant physiology · 2021Article
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. In an attempt to unravel the microalgal triacylglycerol (TAG) pathway, we overexpressed two key lipogenic genes, glycerol-3-phosphate acyltransferase (GPAT1) and lysophosphatidic acid acyltransferase (LPAT1), in oleaginous
resultsEngineered
conclusionOverexpression of GPAT1 together with LPAT1 significantly enhanced lipid content without affecting growth and photosynthetic efficiency, and they orchestrated the expression of other key photosynthetic and lipogenic genes. The lipid profile for elevated fatty acid content (C16-CoA) demonstrated the involvement of the prokaryotic TAG pathway in marine diatoms. The results suggested that engineering dual metabolic nodes should be possible in microalgal lipid metabolism. This study also provides the first demonstration of the role of the prokaryotic TAG biosynthetic pathway in lipid overproduction and indicates that the fatty acid profile can be tailored to improve lipid production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.