ArticleBiotechnology progress2019
A protocol to transfer a fed-batch platform process into semi-perfusion mode: The benefit of automated small-scale bioreactors compared to shake flasks as scale-down model.
Article in Biotechnology progress, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Sustainable biodiesel production from agricultural lignocellulosic waste via oleaginous microbial processes.BMC biotechnology · 2025Review
- Large-Scale Expansion of Suspension Cells in an Automated Hollow-Fiber Perfusion Bioreactor.Bioengineering (Basel, Switzerland) · 2025Article
- Perfusion Process Intensification for Lentivirus Production Using a Novel Scale-Down Model.Biotechnology and bioengineering · 2025Article
- Triple Space-Time Yield in Discontinuous Antibody Biomanufacturing by Combination of Synergetic Process Intensification Strategies.Bioengineering (Basel, Switzerland) · 2023Article
- Online deployment of an O-PLS model for dielectric spectroscopy-based inline monitoring of viable cell concentrations in Chinese hamster ovary cell perfusion cultivations.Engineering in life sciences · 2023Article
- Establishment of a Perfusion Process with Antibody-Producing CHO Cells Using a 3D-Printed Microfluidic Spiral Separator with Web-Based Flow Control.Bioengineering (Basel, Switzerland) · 2023Article
- Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies.Engineering in life sciences · 2023Article
- Boosting Productivity for Advanced Biomanufacturing by Re-Using Viable Cells.Frontiers in bioengineering and biotechnology · 2023Article
- A novel hybrid bioprocess strategy addressing key challenges of advanced biomanufacturing.Frontiers in bioengineering and biotechnology · 2023Article
- Optimization of a mAb production process with regard to robustness and product quality using quality by design principles.Engineering in life sciences · 2022Article
- Stress-induced increase of monoclonal antibody production in CHO cells.Engineering in life sciences · 2022Article
- Rapid intensification of an established CHO cell fed-batch process.Biotechnology progress · 2022Article
- A Novel Approach for Non-Invasive Continuous In-Line Control of Perfusion Cell Cultivations by Raman Spectroscopy.Frontiers in bioengineering and biotechnology · 2022Article
- Model-based intensification of CHO cell cultures: One-step strategy from fed-batch to perfusion.Frontiers in bioengineering and biotechnology · 2022Article
- Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity.Metabolic engineering communications · 2021Article
- Implementation of QbD strategies in the inoculum expansion of a mAb production process.Engineering in life sciences · 2021Article
- Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development.Frontiers in bioengineering and biotechnology · 2019Review
- N-1 semi-continuous transient perfusion in shake flask for ultra-high density seeding of CHO cell cultures in benchtop bioreactors.Biotechnology progressArticle
- Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process.Biotechnology progressArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Continuous processes such as perfusion processes can offer advantages compared to fed-batch or batch processes in bio-processing: improved product quality (e.g. for labile products), increased product yield, and cost savings. In this work, a semi-perfusion process was established in shake flasks and transferred to an automated small-scale bioreactor by daily media exchange via centrifugation based on an existing fed-batch process platform. At first the development of a suitable medium and feed composition, the glucose concentration required by the cells and the cell-specific perfusion rate were investigated in shake flasks as the conventional scale-down system. This lead to an optimized process with a threefold higher titer of 10 g/L monoclonal antibody compared to the standard fed-batch. To proof the suitability and benefit as a small-scale model, the established semi-perfusion process was transferred to an automated small-scale bioreactor with improved pH and dissolved oxygen control. The average specific productivity improved from 24.16 pg/(c*d) in the fed-batch process and 36.04 pg/c*d in the semi-perfusion shake flask to 38.88 pg/(c*d) in the semi-perfusion process performed in the controlled small-scale bioreactor, thus illustrating the benefits resulting from the applied semi-perfusion approach, especially in combination with controlled DO and pH settings. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2757, 2019.
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