ReviewPhotosynthesis research2019
Time-resolved fluorescence measurements on leaves: principles and recent developments.
Review in Photosynthesis research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Improved state‑transition analysis in tobacco lines differing in isoprene emission via spectral‑retrieval separation of PSI and PSII fluorescence at physiological temperature.Photosynthesis research · 2026Article
- Loss of qE Does Not Necessarily Lead to Photoinhibition: Sustained Non-Photochemical Quenching in the Absence of PsbS and Zeaxanthin.Plant, cell & environment · 2026Article
- Functional divergence of LhcSR and PsbS in zeaxanthin-mediated non-photochemical quenching.The New phytologist · 2026Article
- Resolving the contributions of photosystem II, photosystem I, and phycobilisomes to the in vivo fluorescence yield in the red alga Cyanidioschyzon merolae.Photosynthesis research · 2026Article
- Probing Femtosecond Charge Transfer Dynamics in P3HT-WSACS omega · 2025Article
- Excitation spillover from PSII to PSI measured in leaves at 77 K.Plant & cell physiology · 2025Article
- In vivo two-photon FLIM resolves photosynthetic properties of maize bundle sheath cells.Photosynthesis research · 2025Article
- Insights from CaPhotosynthesis research · 2024Review
- Excitation transfer and quenching in photosystem II, enlightened by carotenoid triplet state in leaves.Photosynthesis research · 2024Article
- A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos.Scientific reports · 2024Article
- Immunoassay System Based on the Technology of Time-Resolved Fluorescence Resonance Energy Transfer.Sensors (Basel, Switzerland) · 2024Article
- Light-induced changes of far-red excited chlorophyll fluorescence: further evidence for variable fluorescence of photosystem I in vivo.Photosynthesis research · 2023Article
- Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts.BBA advances · 2023Article
- Fabricated Flexible Composite for a UV-LED Color Filter and Anti-Counterfeiting Application of Calcium Molybdate Phosphor Synthesized at Room Temperature.Materials (Basel, Switzerland) · 2022Article
- Photosynthesis dynamics and regulation sensed in the frequency domain.Plant physiology · 2021Article
- Evidence for variable chlorophyll fluorescence of photosystem I in vivo.Photosynthesis research · 2021Article
- The role of Cytochrome bPhotosynthesis research · 2021Article
- Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Simultaneously measuring pulse-amplitude-modulated (PAM) chlorophyll fluorescence of leaves at wavelengths shorter and longer than 700 nm.Photosynthesis research · 2021Article
- Acclimation of Chlamydomonas reinhardtii to extremely strong light.Photosynthesis research · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Photosynthesis starts when a pigment in the photosynthetic antennae absorbs a photon. The electronic excitation energy is then transferred through the network of light-harvesting pigments to special chlorophyll (Chl) molecules in the reaction centres, where electron transfer is initiated. Energy transfer and primary electron transfer processes take place on timescales ranging from femtoseconds to nanoseconds, and can be monitored in real time via time-resolved fluorescence spectroscopy. This method is widely used for measurements on unicellular photosynthetic organisms, isolated photosynthetic membranes, and individual complexes. Measurements on intact leaves remain a challenge due to their high structural heterogeneity, high scattering, and high optical density, which can lead to optical artefacts. However, detailed information on the dynamics of these early steps, and the underlying structure-function relationships, is highly informative and urgently required in order to get deeper insights into the physiological regulation mechanisms of primary photosynthesis. Here, we describe a current methodology of time-resolved fluorescence measurements on intact leaves in the picosecond to nanosecond time range. Principles of fluorescence measurements on intact leaves, possible sources of alterations of fluorescence kinetics and the ways to overcome them are addressed. We also describe how our understanding of the organisation and function of photosynthetic proteins and energy flow dynamics in intact leaves can be enriched through the application of time-resolved fluorescence spectroscopy on leaves. For that, an example of a measurement on Zea mays leaves is presented.
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