Evidence map›Paper›PMID 30478514›Full record

ArticleAnalytical and bioanalytical chemistry2019

Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides.

He Chen, Qian Yang, Yuan Ding, Natalia Vasylieva, Candace S Bever, Xiude Hua, Minghua Wang, Bruce D Hammock

Open access · greenAbstract read
In one paragraph

Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Article
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  6. Exosomes as Actively Targeted Nanocarriers for Cancer Therapy.International journal of nanomedicine · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

He ChenCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Qian YangCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Yuan DingCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Natalia VasylievaDepartment of Entomology and UCD Cancer Center, University of California, 96 Briggs Hall, Davis, CA, 95616, USA.
Candace S BeverFoodborne Toxin Detection and Prevention Unit, Agricultural Research Service, United States Department of Agriculture, Western Regional Research Center, 800 Buchanan Street, Albany, CA, 94710, USA.
Xiude HuaCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China. huaxiude@njau.edu.cn.
Minghua WangCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Bruce D HammockDepartment of Entomology and UCD Cancer Center, University of California, 96 Briggs Hall, Davis, CA, 95616, USA.
Nanjing Agricultural University · CNUC Davis Comprehensive Cancer CenterUniversity of California, Davis · USWestern Regional Research Center · US

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
Fundamental Research Funds for the Central Universities KYZ201618National Institute of Environmental Health Sciences, Superfund Research Program P42 ES04699National Key Research and Development Program of China 2017YFF0210200NIEHS NIH HHS P42 ES004699
6 · The paper itself

Abstract

Phage-displayed peptides have been proven to be powerful reagents for competitive and noncompetitive immunoassays. However, they are unconventional reagents, which greatly limit their analytical commercial applications and require additional reagents for detection. In this work, the peptides that specifically bind with anti-benzothiostrobin monoclonal antibody (mAb) or benzothiostrobin-mAb immunocomplex were synthesized and conjugated with fluorescein isothiocyanate (FITC) as substitutes of the phage-displayed peptides to avoid their shortcomings and extend their applications. Competitive and noncompetitive fluorescence immunoassays (FIAs) for benzothiostrobin were developed by mAb coupling with magnetic nanoparticles as concentration elements and peptides conjugated with FITC as tracers. Compared with enzyme-linked immunosorbent assays, the FIAs reduced the number of steps from 6 to 2 and analysis time from more than 5 to 1.2 h. The competitive FIA showed the half-maximal inhibition concentration (IC

Indexed as

AcrylatesAntibodies, MonoclonalBenzothiazolesFluorescein-5-isothiocyanateImmunoassayMolecular StructurePeptidesReproducibility of ResultsSensitivity and SpecificityAcrylatesAntibodies, MonoclonalBenzothiazolesbenzothiostrobinFluorescein-5-isothiocyanatePeptidesBenzothiostrobinImmunoassayImmunocomplexPeptidomimeticPesticide residue

Identifiers

PMID30478514
PMCPMC7263471
OpenAlexW2902144353

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.