ArticleScientific reports2018
Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Extracellular matrix mediates circulating tumor cell clustering in triple-negative breast cancer metastasis.Nature communications · 2026Article
- Microfluidics and nanofluidics in India - some recent advancements and futuristic perspective.Biomicrofluidics · 2025Article
- Deformation under flow and morphological recovery of cancer cells.Lab on a chip · 2024Article
- Nuclear rupture induced by capillary constriction forces promotes differential effects on metastatic and normal breast cells.Scientific reports · 2024Article
- Experimental measurement and numerical modeling of deformation behavior of breast cancer cells passing through constricted microfluidic channels.Microsystems & nanoengineering · 2024Article
- Synergistic celecoxib and dimethyl-celecoxib combinations block cervix cancer growth through multiple mechanisms.PloS one · 2024Article
- Microfluidics engineering towards personalized oncology-a review.In vitro models · 2023Review
- On-chip modeling of tumor evolution: Advances, challenges and opportunities.Materials today. Bio · 2023Review
- Article
- Mechanobiology and survival strategies of circulating tumor cells: a process towards the invasive and metastatic phenotype.Frontiers in cell and developmental biology · 2023Review
- On the effectiveness of proton boron fusion therapy (PBFT) at cellular level.Scientific reports · 2022Article
- Article
- Circulating prostate cancer cells have differential resistance to fluid shear stress-induced cell death.Journal of cell science · 2021Article
- Review
- Squeezing through the microcirculation: survival adaptations of circulating tumour cells to seed metastasis.British journal of cancer · 2021Review
- Improving viability of leukemia cells by tailoring shell fluid rheology in constricted microcapillary.Scientific reports · 2020Article
- Fluid shear stress coupled with narrow constrictions induce cell type-dependent morphological and molecular changes in SK-BR-3 and MDA-MB-231 cells.Scientific reports · 2020Article
- Deciphering Hydrodynamic and Drug-Resistant Behaviors of Metastatic EMT Breast Cancer Cells Moving in a Constricted Microcapillary.Journal of clinical medicine · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the burgeoning challenges of metastasis, a microchannel of 35 μm diameter, constricted to 7 μm for a distance of 200 μm in a total length of 3 mm, was designed and fabricated using a mask aligner made of polydimethylsiloxane (PDMS) to mimic in vivo capillaries. A thin glass cover-slide was mounted on top to monitor the motion of single or aggregated malignant HeLa cells (size 17-30 μm) microscopically through the constricted microchannel at a constant flow rate of 30 μl/h. Quantitative deconvolution of high-speed videographs of a single cell of 30 μm revealed cellular deformation while passing through constriction, having elongation index, average transit velocity and entry time of 2.67, 18 mm/s and 5.1 ms, respectively. Morphological analysis of live and apoptotic cells by dual staining with Acridine Orange/Ethidium Bromide demonstrated retention of a significant viable cell population after exit through the constriction and a viability index of 50% was quantified by dye exclusion assay. The cumulative data for microfluidic parameters, morphology and relevant metastatic MMP2 gene expression efficiency measured by real-time polymerase chain reaction revealed retention of virulence potency that could possibly cause metastasis, would be beneficial in developing futuristic MEMS device for cancer theranostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.